College of Life Sciences, Henan Normal University, Xinxiang 453007, China.
State Key Laboratory of Marine Environmental Science, School of Life Sciences, Xiamen University, Xiamen 361005, China.
Bioresour Technol. 2017 Sep;239:137-143. doi: 10.1016/j.biortech.2017.05.028. Epub 2017 May 8.
In this study, an actinomycete Streptomyces sp. hsn06 with the ability to harvest Chlorella vulgaris biomass was used to investigate the flocculation mechanism. Streptomyces sp. hsn06 exhibited flocculation activity on algal cells through mycelial pellets with adding calcium. Calcium was determined to promote flocculation activity of mycelial pellets as a bridge binding with mycelial pellets and algal cells, which implied that calcium bridging is the main flocculation mechanism for mycelial pellets. Characteristics of flocculation activity confirmed proteins in mycelial pellets involved in flocculation procedure. The morphology and structure of mycelial pellets also caused dramatic effects on flocculation activity of mycelial pellets. According to the results, Streptomyces sp. hsn06 can be used as a novel flocculating microbial resource for high-efficiency harvesting of microalgae biomass.
在这项研究中，使用了一种具有收集小球藻生物质能力的放线菌链霉菌 hsn06 来研究絮凝机制。链霉菌 hsn06 通过添加钙离子的菌丝球对藻类细胞表现出絮凝活性。钙被确定为促进菌丝球的絮凝活性，作为与菌丝球和藻类细胞结合的桥梁，这表明钙桥联是菌丝球絮凝的主要机制。絮凝活性的特性证实了菌丝球中的蛋白质参与了絮凝过程。菌丝球的形态和结构也对菌丝球的絮凝活性产生了显著影响。根据研究结果，链霉菌 hsn06 可用作一种新型的絮凝微生物资源，用于高效收获微藻生物质。
