Chemical Engineering Laboratory, Faculty of Sciences and Centre for Advanced Scientific Research (CICA), University of La Coruña, Rúa da Fraga 10, E - 15008 A Coruña, Spain.
Chemical Engineering Laboratory, Faculty of Sciences and Centre for Advanced Scientific Research (CICA), University of La Coruña, Rúa da Fraga 10, E - 15008 A Coruña, Spain.
Bioresour Technol. 2017 Sep;239:244-249. doi: 10.1016/j.biortech.2017.05.019. Epub 2017 May 5.
Syngas bioconversion is a promising method for bioethanol production, but some VFA remains at the end of fermentation. A two-stage process was set-up, including syngas fermentation as first stage under strict anaerobic conditions using C. autoethanogenum as inoculum, with syngas (CO/CO/H/N, 30/10/20/40) as gaseous substrate. The second stage consisted in various fed-batch assays using a highly enriched PHA accumulating biomass as inoculum, where the potential for biopolymer production from the remaining acetic acid at the end of the syngas fermentation was evaluated. All of the acetic acid was consumed and accumulated as biopolymer, while ethanol and 2,3-butanediol remained basically unused. It can be concluded that a high C/N ratio in the effluent from the syngas fermentation stage was responsible for non-consumption of alcohols. A maximum PHA content of 24% was reached at the end of the assay.
合成气生物转化是生产生物乙醇的一种很有前途的方法，但发酵结束时仍会有一些 VFA 残留。建立了两段式工艺，包括使用 C. autoethanogenum 作为接种物在严格的厌氧条件下进行的合成气发酵第一阶段，气态底物为合成气（CO/CO/H/N，30/10/20/40）。第二阶段包括使用高度富集的 PHB 积累生物质作为接种物的各种分批补料实验，评估了从合成气发酵结束时剩余的乙酸生产生物聚合物的潜力。所有的乙酸都被消耗并积累为生物聚合物，而乙醇和 2,3-丁二醇基本上未被使用。可以得出结论，合成气发酵阶段流出物中的高 C/N 比是导致醇类未被消耗的原因。在实验结束时达到了 24%的最大 PHB 含量。
