Lubin Arnaud, De Vries Ronald, Cabooter Deirdre, Augustijns Patrick, Cuyckens Filip
Discovery Sciences, Janssen R&D, Beerse, Belgium.
Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, Leuven, Belgium.
J Pharm Biomed Anal. 2017 Aug 5;142:225-231. doi: 10.1016/j.jpba.2017.05.003. Epub 2017 May 4.
The type and design of an ionization source can have a significant influence on the performances of a bioanalytical method. It is, therefore, of high interest to evaluate the performances of newly introduced sources to highlight their benefits and limitations in comparison to other well established sources. In this paper, liquid chromatography - mass spectrometry (LC/MS) performances of a new atmospheric pressure ionization (API) source, commercialized as UniSpray, is evaluated. The dynamic range of 24 pharmaceutical and biological compounds is compared between the new API source and electrospray ionization (ESI) for 3 different mobile phase conditions. Matrix effects are also compared with ESI on a refined selection of 19 pharmaceutical and biological compounds in 4 matrices commonly encountered in bioanalysis. A slightly better dynamic range towards lower concentrations was often observed with the new API source. Matrix effects were quite similar between the two sources with a small, but statistically significant, lower percentage of matrix effects observed for the new API source in plasma and bile in the positive ion mode, and bile in negative ion mode for ESI. Finally, the sensitivity of late eluting compounds could be improved on the new API source by post-column addition of water.
离子源的类型和设计会对生物分析方法的性能产生重大影响。因此，评估新引入的离子源的性能，以突出其与其他成熟离子源相比的优势和局限性，具有很高的研究价值。本文对一种名为UniSpray的新型大气压电离（API）源的液相色谱-质谱联用（LC/MS）性能进行了评估。在3种不同的流动相条件下，比较了新型API源和电喷雾电离（ESI）对24种药物和生物化合物的动态范围。还在生物分析中常见的4种基质中，对19种药物和生物化合物进行了精细筛选，比较了新型API源与ESI的基质效应。新型API源在较低浓度下的动态范围通常略好。两种离子源的基质效应相当相似，在正离子模式下，新型API源在血浆和胆汁中的基质效应百分比略低，但具有统计学意义，在负离子模式下，ESI在胆汁中的基质效应百分比略低。最后，通过柱后加水可以提高新型API源上晚洗脱化合物的灵敏度。
