Shirinifard Abbas, Thiagarajan Suresh, Johnson Melissa D, Calabrese Christopher, Sablauer András
Department of Information Sciences, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
Department of Pathology, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
Ultrasound Med Biol. 2017 Aug;43(8):1628-1638. doi: 10.1016/j.ultrasmedbio.2017.02.004. Epub 2017 May 15.
We investigated the feasibility of estimating absolute tissue blood perfusion using dynamic contrast-enhanced ultrasound (CEUS) imaging in mice. We developed a novel method of microbubble administration and a model-free approach to estimate absolute kidney perfusion, and explored the kidney as a reference organ to estimate absolute perfusion of a neuroblastoma tumor. We performed CEUS on the kidneys of CD1 nude mice using the VisualSonics VEVO 2100 imaging system. We estimated individual kidney blood perfusion using the burst-replenishment (BR) technique. We repeated the kidney imaging on the mice after a week. We performed CEUS imaging of a neuroblastoma mouse xenograft tumor along with its right kidney using two sets of microbubble administration parameters to estimate absolute tumor blood perfusion. We performed statistical tests at a significance level of 0.05. Our estimated absolute kidney perfusion (425 ± 123 mL/min/100 g) was within the range of previously reported values. There was no statistical difference between the estimated absolute kidney blood perfusions from the 2 wk of imaging (paired t-test, p = 0.09). We estimated the absolute blood perfusion in the neuroblastoma tumor to be 16.49 and 16.9 mL/min/100 g for the two sets of microbubble administration parameters (Wilcoxon rank-sum test, p = 0.6). We have established the kidney as a reliable reference organ in which to estimate absolute perfusion of other tissues. Using a neuroblastoma tumor, we have determined the feasibility of estimating absolute blood perfusion in tissues using contrast-enhanced ultrasound imaging.
我们研究了使用动态对比增强超声（CEUS）成像估计小鼠绝对组织血流灌注的可行性。我们开发了一种新型微泡给药方法和一种无模型方法来估计绝对肾脏灌注，并探索将肾脏作为参考器官来估计神经母细胞瘤肿瘤的绝对灌注。我们使用VisualSonics VEVO 2100成像系统对CD1裸鼠的肾脏进行了CEUS检查。我们使用爆破-再充盈（BR）技术估计个体肾脏血流灌注。一周后，我们对小鼠重复进行肾脏成像。我们使用两组微泡给药参数对神经母细胞瘤小鼠异种移植瘤及其右肾进行CEUS成像，以估计肿瘤绝对血流灌注。我们在显著性水平为0.05的情况下进行了统计检验。我们估计的绝对肾脏灌注（425±123 mL/min/100 g）在先前报道的值范围内。两次成像周的估计绝对肾脏血流灌注之间无统计学差异（配对t检验，p = 0.09）。对于两组微泡给药参数，我们估计神经母细胞瘤肿瘤的绝对血流灌注分别为16.49和16.9 mL/min/100 g（Wilcoxon秩和检验，p = 0.6）。我们已将肾脏确立为估计其他组织绝对灌注的可靠参考器官。通过神经母细胞瘤肿瘤，我们确定了使用对比增强超声成像估计组织中绝对血流灌注的可行性。
