Ball J A, Burnet P W, Bretherton-Watt D, Bloom S R
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.
J Neurol Sci. 1988 Dec;88(1-3):177-84. doi: 10.1016/0022-510x(88)90215-8.
In Huntington's disease (HD), reduction in striatal GABA is one of the most striking abnormalities and alterations in benzodiazepine receptors, which are allosterically linked to the GABAA receptor, have also been reported. Diazepam binding inhibitor (DBI), recently isolated from rat and human brain, has been proposed as an endogenous ligand at the benzodiazepine receptor. The content of DBI-like immunoreactivity(51-70) (DBI-IR(51-70), has therefore been compared in control postmortem human brains and in HD brains (matched for age, sex and post-mortem delay), using a specific radioimmunoassay. DBI-IR(51-70) was more than 1.5-fold increased in the putamen, caudate, globus pallidus and nucleus accumbens of HD brains compared to the control group (P less than 0.001). Gel filtration chromatography showed similar elution profiles of the peptide in both control and HD extracts, thus providing no evidence for a change in the nature of the peptide itself.
在亨廷顿舞蹈病（HD）中，纹状体γ-氨基丁酸（GABA）减少是最显著的异常之一，并且也有报道称与GABAA受体变构连接的苯二氮䓬受体发生了改变。最近从大鼠和人脑中分离出的地西泮结合抑制剂（DBI），被认为是苯二氮䓬受体的内源性配体。因此，使用特异性放射免疫分析法，比较了对照死后人类大脑和HD大脑（年龄、性别和死后延迟时间匹配）中DBI样免疫反应性（51 - 70）（DBI-IR(51-70)）的含量。与对照组相比，HD大脑的壳核、尾状核、苍白球和伏隔核中DBI-IR(51-70)增加了1.5倍以上（P小于0.001）。凝胶过滤色谱显示，对照提取物和HD提取物中该肽的洗脱曲线相似，因此没有证据表明该肽本身的性质发生了变化。
