• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Sialyltransferase-1 in a human malignant glioma cell line. Kinetic characteristics and effect of human interferon-beta.

作者信息

Yates A J, Collins J F, Elder P J, Stephens R E

机构信息

Department of Pathology, College of Medicine, Ohio State University, Columbus 43210.

出版信息

J Neurooncol. 1988 Sep;6(2):179-84. doi: 10.1007/BF02327394.

DOI:10.1007/BF02327394
PMID:2852219
Abstract

Sialyltransferase-1 activity was studied in cultured 12-18 human glioma cells. The apparent Km and Vmax with variable LacCer concentrations were 32 microM and 197 pmoles/mg protein/hr and with variable CMP-NeuAc concentrations were 172 microM and 877 pmoles/mg protein/hr., respectively. The pH optimum towards exogenous LacCer was 6.0 and towards endogenous acceptors was 6.2. The optimum protein:detergent ratio was 1:1. Human beta interferon (1000 units/ml medium) increased sialyltransferase-1 activity only slightly on a protein basis but increased it 47% on a per cell basis. These results demonstrate that one of the biochemical effects of beta-interferon on 12-18 human glioma cells is to stimulate ganglioside synthesis.

摘要

相似文献

1
Sialyltransferase-1 in a human malignant glioma cell line. Kinetic characteristics and effect of human interferon-beta.
J Neurooncol. 1988 Sep;6(2):179-84. doi: 10.1007/BF02327394.
2
Effects of lectin activation on sialyltransferase activities in human lymphocytes.凝集素激活对人淋巴细胞唾液酸转移酶活性的影响。
Biochemistry. 1986 May 6;25(9):2577-81. doi: 10.1021/bi00357a044.
3
Regulation of sialyltransferase activities by phosphorylation and dephosphorylation.唾液酸转移酶活性的磷酸化和去磷酸化调节
J Neurochem. 1995 May;64(5):2295-302. doi: 10.1046/j.1471-4159.1995.64052295.x.
4
Growth inhibition of cultured human glioma cells by beta-interferon is not dependent on changes in ganglioside composition.β-干扰素对培养的人胶质瘤细胞的生长抑制作用不依赖于神经节苷脂组成的变化。
J Neuropathol Exp Neurol. 1988 Mar;47(2):119-27. doi: 10.1097/00005072-198803000-00004.
5
Bovine colostrum CMP-NeuAc:Gal beta(1-->4)GlcNAc-R alpha(2-->6)-sialyltransferase is involved in the synthesis of the terminal NeuAc alpha(2-->6)GalNAc beta(1-->4)GlcNAc sequence occurring on N-linked glycans of bovine milk glycoproteins.牛初乳CMP-唾液酸：β-半乳糖（1→4）-N-乙酰葡糖胺-R α（2→6）-唾液酸转移酶参与了牛乳糖蛋白N-连接聚糖上末端唾液酸α（2→6）-N-乙酰半乳糖胺β（1→4）-N-乙酰葡糖胺序列的合成。
Biochem J. 1992 Oct 1;287 ( Pt 1)(Pt 1):311-6. doi: 10.1042/bj2870311.
6
Characterization of a CMP-sialic acid:lactosylceramide sialyltransferase activity in cultured hamster cells.培养的仓鼠细胞中CMP-唾液酸：乳糖基神经酰胺唾液酸转移酶活性的鉴定
Biochim Biophys Acta. 1984 Aug 17;804(4):442-9. doi: 10.1016/0167-4889(84)90072-7.
7
A highly sensitive fluorometric assay for sialyltransferase activity using CMP-9-fluoresceinyl-NeuAc as donor.
Anal Biochem. 1990 Apr;186(1):127-34. doi: 10.1016/0003-2697(90)90585-w.
8
[Sialyltransferase in human malignant melanoma].
Clin Chim Acta. 1976 Oct 1;72(1):3308. doi: 10.1016/0009-8981(76)90034-6.
9
Characterization of two glycolipid: alpha 2-3sialyltransferases, SAT-3 (CMP-NeuAc:nLcOse4Cer alpha 2-3sialyltransferase) and SAT-4 (CMP-NeuAc:GgOse4Cer alpha 2-3sialyltransferase), from human colon carcinoma (Colo 205) cell line.来自人结肠癌细胞系（Colo 205）的两种糖脂：α2-3唾液酸转移酶，SAT-3（CMP-神经氨酸：nLcOse4Cer α2-3唾液酸转移酶）和SAT-4（CMP-神经氨酸：GgOse4Cer α2-3唾液酸转移酶）的特性鉴定
Biochemistry. 1996 Apr 23;35(16):5166-74. doi: 10.1021/bi960239l.
10
Transfer of synthetic sialic acid analogues to N- and O-linked glycoprotein glycans using four different mammalian sialyltransferases.使用四种不同的哺乳动物唾液酸转移酶将合成唾液酸类似物转移至N-和O-连接的糖蛋白聚糖上。
Biochemistry. 1989 Sep 5;28(18):7386-92. doi: 10.1021/bi00444a036.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Gangliosides of human cerebral astrocytomas.人类脑星形细胞瘤的神经节苷脂
J Neurochem. 1980 Jan;34(1):126-31. doi: 10.1111/j.1471-4159.1980.tb04630.x.
3
Biosynthesis in vitro of sialyl(alpha 2-3)neolactotetraosylceramide by a sialyltransferase from embryonic chicken brain.
J Biol Chem. 1982 Nov 10;257(21):12765-9.
4
Cell surface glycolipids and glycoproteins in malignant transformation.恶性转化中的细胞表面糖脂和糖蛋白。
Adv Cancer Res. 1983;38:289-350. doi: 10.1016/s0065-230x(08)60191-8.
5
Biology of gangliosides: neuritogenic and neuronotrophic properties.神经节苷脂的生物学：促神经突生长和神经营养特性。
J Neurosci Res. 1984;12(2-3):147-59. doi: 10.1002/jnr.490120204.
6
Growth characteristics of human glioma-derived and fetal neural cells in culture.人胶质瘤来源细胞和胎儿神经细胞在培养中的生长特性
J Neuropathol Exp Neurol. 1981 Sep;40(5):512-25. doi: 10.1097/00005072-198109000-00003.
7
Correlation between ganglioside distribution and histological grading of human astrocytomas.
Int J Cancer. 1985 Sep 15;36(3):363-6.
8
Effects of lectin activation on sialyltransferase activities in human lymphocytes.凝集素激活对人淋巴细胞唾液酸转移酶活性的影响。
Biochemistry. 1986 May 6;25(9):2577-81. doi: 10.1021/bi00357a044.
9
Growth inhibition of cultured human glioma cells by beta-interferon is not dependent on changes in ganglioside composition.β-干扰素对培养的人胶质瘤细胞的生长抑制作用不依赖于神经节苷脂组成的变化。
J Neuropathol Exp Neurol. 1988 Mar;47(2):119-27. doi: 10.1097/00005072-198803000-00004.
10
Effects of interferon and gangliosides on growth of cultured human glioma and fetal brain cells.干扰素和神经节苷脂对培养的人胶质瘤细胞和胎儿脑细胞生长的影响。
Cancer Res. 1985 Mar;45(3):1033-9.