Wang Lin-Fang, Huang Shu-Bing, Zhao Hou-De, Liu Chun-Jie, Yao Li, Shen Yan-Qin
Medical School, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
Medical School, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; Center for Neuroscience, Shantou University Medical College, 22 Xinling Road, Shantou, Guangdong 515041, China.
Biochem Biophys Res Commun. 2017 Jul 1;488(3):522-527. doi: 10.1016/j.bbrc.2017.05.079. Epub 2017 May 15.
Zebrafish is an excellent model to study the mechanisms underlying successful central nervous system (CNS) regeneration. Previous study shows that activating transcription factor 3 (ATF3) promotes neurite outgrowth and is involved in optic nerve regeneration in zebrafish. Here, we used zebrafish model to investigate the role of ATF3 in regeneration following spinal cord injury (SCI). Quantitative polymerase chain reaction (qPCR) and in situ hybridization revealed that ATF3 mRNA levels increased at 12 h and 6 d following SCI. Double labeled immunofluorescence showed that ATF3 expressed in motoneurons. Treatment of anti-sense ATF3 morpholino (MO) inhibited locomotor recovery and decreased axon regeneration of spinal cord injured zebrafish. Further, inhibition of ATF3 up-regulated the expression of inflammatory factors tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β). These data suggest that ATF3 could promote locomotor recovery and axon regrowth in zebrafish SCI model possibly by regulating inflammatory response.
斑马鱼是研究成功的中枢神经系统（CNS）再生机制的优秀模型。先前的研究表明，激活转录因子3（ATF3）可促进神经突生长，并参与斑马鱼的视神经再生。在此，我们使用斑马鱼模型来研究ATF3在脊髓损伤（SCI）后再生中的作用。定量聚合酶链反应（qPCR）和原位杂交显示，SCI后12小时和6天时ATF3 mRNA水平升高。双标免疫荧光显示ATF3在运动神经元中表达。用反义ATF3吗啉代寡核苷酸（MO）处理可抑制脊髓损伤斑马鱼的运动恢复并减少轴突再生。此外，抑制ATF3会上调炎症因子肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）的表达。这些数据表明，ATF3可能通过调节炎症反应来促进斑马鱼SCI模型中的运动恢复和轴突再生。
