Sarawar S R, Schlingemann R O, Kelsey A, Fleming S, Kumar S
Clinical Research Laboratories, Christie Hospital, Manchester, U.K.
J Pathol. 1988 Dec;156(4):319-24. doi: 10.1002/path.1711560408.
The monoclonal antibody PAL-E is specific for endothelial cells in a wide variety of normal and tumour tissue. In normal kidney, PAL-E reacts exclusively with the endothelium of non-glomerular blood vessels. In Wilms' tumour, binding of PAL-E was not restricted to the endothelium; staining of blastemal cells was observed in seven out of eight cases examined. Mesenchymal and tubular components, if present in Wilms' tumour, were negative. In contrast, a monoclonal antibody to Factor VIII-related antigen (RFF-8-R-1) bound only to endothelial cells in these tumours. In fetal kidney, PAL-E binding showed a wider distribution than in adult kidney and both stromal and glomerular capillaries were stained. Tubules and non-endothelial stromal cells were negative. These results indicate that the reactivity of the monoclonal antibody, PAL-E, is not restricted to cells of endothelial origin in all tissues. The implications of these findings for the differentiation of Wilms' tumour are discussed.
单克隆抗体PAL-E对多种正常组织和肿瘤组织中的内皮细胞具有特异性。在正常肾脏中，PAL-E仅与非肾小球血管的内皮细胞发生反应。在肾母细胞瘤中，PAL-E的结合并不局限于内皮细胞；在八例检查病例中的七例中观察到胚基细胞染色。如果肾母细胞瘤中存在间充质和肾小管成分，则呈阴性。相比之下，一种针对VIII因子相关抗原的单克隆抗体（RFF-8-R-1）仅与这些肿瘤中的内皮细胞结合。在胎儿肾脏中，PAL-E结合的分布比成人肾脏更广泛，基质和肾小球毛细血管均被染色。肾小管和非内皮基质细胞呈阴性。这些结果表明，单克隆抗体PAL-E的反应性在所有组织中并不局限于内皮起源的细胞。讨论了这些发现对肾母细胞瘤分化的意义。
