大鼠肾上腺皮质中谷胱甘肽过氧化物酶（GPx1）的免疫定位：类固醇生成与脂质过氧化之间的相关性
Immunolocalization of Glutathione-Peroxidase (GPx1) in the Rat Adrenal Cortex: Correlation between Steroidogenesis and Lipid Peroxidation.
作者信息
Murakoshi Masanori, Osamura Robert Yoshiyuki
机构信息
ASKA Pharmaceutical Co., Ltd, 5-36-1, Shimosakunobe, Takatsu-ku, Kawasaki 213-8522, Japan.
Sanno Hospital, 8-10-16, Akasaka, Minato-ku, Tokyo 107-0052, Japan.
出版信息
Acta Histochem Cytochem. 2017 Apr 27;50(2):57-61. doi: 10.1267/ahc.17010. Epub 2017 Apr 15.
In order to confirm the relationship between glutathione-peroxidase (GPx1) and biological significance on steroidogenesis, we have studied the immunocytochemical localization of GPx1 in the rat adrenal cortical cells. GPx1 was observed not only in cytoplasm (cytosol GPx1) but in mitochondria (mitochondrial GPx1). The staining intensity was altered by the functional state of the adrenal cortical cells. Furthermore, cytosol- and mitochondrial-GPx1 was modified by lipoperoxidative damage in the adrenal cortical cells. Therefore, we proposed that the pattern of GPx1 staining should be a more sensitive and specific indicator of oxidative damage in cells. Thus, the staining pattern of GPx1 is thought to be a useful marker for lipid peroxidation in the adrenal cortical cells.
为了证实谷胱甘肽过氧化物酶（GPx1）与类固醇生成的生物学意义之间的关系，我们研究了GPx1在大鼠肾上腺皮质细胞中的免疫细胞化学定位。不仅在细胞质（胞质GPx1）中观察到GPx1，在线粒体（线粒体GPx1）中也观察到了。染色强度随肾上腺皮质细胞的功能状态而改变。此外，肾上腺皮质细胞中的胞质和线粒体GPx1会因脂质过氧化损伤而发生改变。因此，我们提出GPx1染色模式应是细胞氧化损伤更敏感和特异的指标。因此，GPx1的染色模式被认为是肾上腺皮质细胞脂质过氧化的有用标志物。
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