猪穹窿下器官中脑钠肽特异性结合位点的受体放射自显影证据。

Receptor autoradiographic evidence of specific brain natriuretic peptide binding sites in the porcine subfornical organ.

作者信息

Niwa M, Shigematsu K, Kurihara M, Kataoka Y, Maeda T, Nakao K, Imura H, Matsuo H, Tsuchiyama H, Ozaki M

机构信息

Department of Pharmacology, Nagasaki University School of Medicine, Japan.

出版信息

Neurosci Lett. 1988 Dec 19;95(1-3):113-8. doi: 10.1016/0304-3940(88)90642-8.

DOI:10.1016/0304-3940(88)90642-8

PMID:2852316

Abstract

Specific binding sites of brain natriuretic peptide (BNP), a newly discovered peptide in the subfornical organ (SFO) of porcine brain were investigated, following incubation of related tissue sections with 125I-BNP, then using autoradiography and an image analysis coupled with computer-assisted microdensitometry. Specific 125I-BNP binding sites were found to be localized in the SFO, an area densely labeled by 125I-alpha-rat atrial natriuretic peptide and 125I-(Sar1,Ile8)-angiotensin II. Specific 125I-BNP binding to the SFO was displaced by unlabeled BNP, with a high affinity, and was calculated to be Ka = 0.385 x 10(-9) M and Bmax = 40.1 fmol/mg using a LIGAND computer program. Acquisition of these present findings enhances our knowledge of the physiology of BNP, atrial natriuretic peptides and angiotensin II system in the SFO.

摘要

利用放射自显影、图像分析及计算机辅助显微密度测定法，研究了脑钠肽（BNP）在猪脑穹窿下器（SFO）中的特异性结合位点。相关组织切片与125I-BNP孵育后，发现125I-BNP特异性结合位点定位于SFO，该区域被125I-α-大鼠心钠素和125I-（Sar1，Ile8）-血管紧张素II密集标记。未标记的BNP可高亲和力取代SFO上特异性结合的125I-BNP，使用LIGAND计算机程序计算得Ka = 0.385×10（-9）M，Bmax = 40.1 fmol/mg。这些研究结果增进了我们对SFO中BNP、心钠素和血管紧张素II系统生理学的认识。