Ghosh Somiranjan, Dutta Sisir, Thorne Gabriel, Boston Ava, Barfield Alexis, Banerjee Narendra, Walker Rayshawn, Banerjee Hirendra Nath
Molecular Genetics Laboratory, Department of Biology, Howard University, Washington, DC 20059, USA.
Department of Natural, Pharmacy and Health Sciences, Elizabeth City State University, University of North Carolina.
Int J Sci Res Sci Eng Technol. 2017 Feb;3(1):458-465. Epub 2017 Feb 1.
Glioblastoma multiforme (GBM) is the most common and aggressive type of the primary brain tumors with pathologic hallmarks of necrosis and vascular proliferation. The diagnosis of GBM is currently mostly based on histological examination of brain tumor tissues, after radiological characterization and surgical biopsy. The ability to characterize tumors comprehensively at the molecular level raises the possibility that diagnosis can be made based on molecular profiling with or without histological examination, rather than solely on histological phenotype. The development of novel genomic and proteomic techniques will foster in the identification of such diagnostic and prognostic molecular markers. We analyzed the global differential gene expression of a GBM cell line HTB15 in comparison to normal human Astrocytes, and established a few canonical pathways that are important in determining the molecular mechanisms of cancer using global gene expression microarray, coupled with the Ingenuity Pathway Analysis (®). Overall, we revealed a discrete gene expression profile in the experimental model that resembled progression of GBM cancer. The canonical pathway analysis showed the involvement of genes that differentially expressed in such a disease condition that included , and . Our findings established that the gene expression pattern of this dreaded brain cancer will probably help the cancer research community by finding out newer therapeutic strategies to combat this dreaded cancer type that leads to the identification of high-risk population in this category, with almost hundred percent mortality rate.
多形性胶质母细胞瘤（GBM）是原发性脑肿瘤中最常见且侵袭性最强的类型，具有坏死和血管增生的病理特征。目前，GBM的诊断主要基于脑肿瘤组织的组织学检查，此前需进行放射学特征分析和手术活检。在分子水平全面表征肿瘤的能力增加了基于分子谱分析进行诊断的可能性，无论是否进行组织学检查，而不仅仅基于组织学表型。新型基因组和蛋白质组技术的发展将有助于鉴定此类诊断和预后分子标志物。我们分析了GBM细胞系HTB15与正常人星形胶质细胞相比的全局差异基因表达，并使用全局基因表达微阵列结合Ingenuity Pathway Analysis（®）建立了一些在确定癌症分子机制中重要的典型通路。总体而言，我们在实验模型中揭示了一种离散的基因表达谱，类似于GBM癌症的进展。典型通路分析表明，在这种疾病状态下差异表达的基因包括 、 和 。我们的研究结果表明，这种可怕的脑癌的基因表达模式可能会通过找出对抗这种可怕癌症类型的新治疗策略来帮助癌症研究界，从而识别出这一类别中的高危人群，其死亡率几乎为百分之百。
