Cîmpeanu Raluca Alexandra, Popescu Dragoş Marian, Burada Florin, Cucu Mihai Gabriel, Gheonea Dan IonuŢ, Ioana Mihai, Rogoveanu Ion
Human Genomics Laboratory, University of Medicine and Pharmacy of Craiova, Romania;
Rom J Morphol Embryol. 2017;58(1):125-129.
Accumulating evidence that microRNA (miRNA) genes are involved in different processes associated with gastric carcinogenesis. The polymorphisms located on miRNA sequences may affect the interaction with their target messenger RNAs (mRNAs) and, consequently, genetic susceptibility to disease. The aim of our study was to investigate the association of miR-149 rs2292832 C>T polymorphism and gastric cancer susceptibility in Romanian patients. A total of 142 patients with gastric adenocarcinoma and 288 healthy controls were included in this study. The miR-149 rs2292832 allelic variants were genotyped by real-time polymerase chain reaction (RT-PCR) using specific TaqMan predesigned probes. The association between polymorphism and gastric cancer risk was estimated by odds ratio (OR) and 95% confidence interval (CI). The miR-149 rs2292832 C>T was not associated with susceptibility to gastric cancer, when TT genotype was compared with the more frequent AA genotype (OR 0.98, 95% CI 0.55-1.77, p=0.96) or when we used dominant and recessive models. Also, we compared allele frequencies and no correlation was found (OR 0.92, 95% CI 0.68-1.24, p=0.57). The sub-classification of gastric cancer into non-cardia and cardia or intestinal and diffuse type did not reveal any statistically significant difference for investigated polymorphism.
越来越多的证据表明，微小RNA（miRNA）基因参与了与胃癌发生相关的不同过程。位于miRNA序列上的多态性可能会影响其与靶信使核糖核酸（mRNA）的相互作用，从而影响疾病的遗传易感性。我们研究的目的是调查罗马尼亚患者中miR-149 rs2292832 C>T多态性与胃癌易感性之间的关联。本研究共纳入了142例胃腺癌患者和288例健康对照。使用特定的TaqMan预设计探针，通过实时聚合酶链反应（RT-PCR）对miR-149 rs2292832等位基因变体进行基因分型。通过比值比（OR）和95%置信区间（CI）评估多态性与胃癌风险之间的关联。当将TT基因型与更常见的AA基因型进行比较时（OR 0.98，95% CI 0.55-1.77，p=0.96），或者当我们使用显性和隐性模型时，miR-149 rs2292832 C>T与胃癌易感性无关。此外，我们比较了等位基因频率，未发现相关性（OR 0.92，95% CI 0.68-1.24，p=0.57）。将胃癌分为非贲门和贲门型或肠型和弥漫型，对于所研究的多态性未显示出任何统计学上的显著差异。
