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植物代谢物的激光烧蚀电感耦合等离子体质谱成像

Laser Ablation Inductively Coupled Plasma Mass Spectrometry Imaging of Plant Metabolites.

作者信息

Seaman Callie

机构信息

Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, City Campus, Howard Street, Sheffield, S1 1WB, UK.

出版信息

Methods Mol Biol. 2017;1618:125-135. doi: 10.1007/978-1-4939-7051-3_11.

Abstract

LA-ICP-MS (Laser Ablation Inductively Coupled Plasma Mass Spectrometry) is a highly sensitive isotopic and elemental analytical technique that has a wide application for solid samples such as plant material. Within this chapter we describe how to prepare leaf material, seeds, and fruit for 2D elemental distribution maps, preparation of reference materials, method condition optimization, special resolution calculation, and the limit of detection.

摘要

激光烧蚀电感耦合等离子体质谱法(LA-ICP-MS)是一种高灵敏度的同位素和元素分析技术,在植物材料等固体样品中有着广泛应用。在本章中,我们将描述如何制备叶片材料、种子和果实以获取二维元素分布图,参考材料的制备,方法条件优化,特殊分辨率计算以及检测限。

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