Guillaumet-Adkins Amy, Heyn Holger
CNAG-CRG, Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST), Universitat Pompeu Fabra (UPF), Parc Científic de Barcelona - Torre I, Baldiri Reixac, 4, Barcelona, Catalonia, 08028, Spain.
CNAG-CRG, Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST), National Centre for Genomic Analysis (CNAG), Parc Científic de Barcelona - Torre I, Baldiri Reixac, 4, Barcelona, Catalonia, 08028, Spain.
Adv Exp Med Biol. 2017;978:393-407. doi: 10.1007/978-3-319-53889-1_20.
The brain is the most complex tissue in terms of cell types that it comprises, to the extent that it is still poorly understood. Single cell genome and transcriptome profiling allow to disentangle the neuronal heterogeneity, enabling the categorization of individual neurons into groups with similar molecular signatures. Herein, we unravel the current state of knowledge in single cell neurogenomics. We describe the molecular understanding of the cellular architecture of the mammalian nervous system in health and in disease; from the discovery of unrecognized cell types to the validation of known ones, applying these state-of-the-art technologies.
就其包含的细胞类型而言，大脑是最复杂的组织，以至于人们对它的了解仍然很少。单细胞基因组和转录组分析能够解开神经元的异质性，从而将单个神经元分类为具有相似分子特征的组。在此，我们揭示单细胞神经基因组学的当前知识状态。我们描述了在健康和疾病状态下，对哺乳动物神经系统细胞结构的分子理解；从发现未被识别的细胞类型到验证已知的细胞类型，应用这些最先进的技术。
