Department of Chemical and Biomolecular Engineering, Yonsei University , Yonsei-ro 50, Seoul 120-749, South Korea.
Department of Radiology, College of Medicine, Yonsei University , Seoul 120-752, South Korea.
ACS Appl Mater Interfaces. 2017 May 31;9(21):17702-17709. doi: 10.1021/acsami.6b16452. Epub 2017 May 19.
Various types of nanoprobes have recently been utilized to monitor living organisms by detecting and imaging intracellular biomarkers, such as microRNAs (miRs). We here present a simple one-pot method to prepare stellate gold nanoparticles functionalized with miR-detecting molecular beacons (SGNP-MBs); low pH conditions permitted the rapid-high loading of MBs on the surface of SGNPs. Compared to the conventional gold nanoparticle-based MBs, SGNPs carried a 4.5-fold higher load of MBs and exhibited a 6.4-fold higher cellular uptake. We demonstrated that SGNP-MBs were successfully internalized in human gastric cancer cell lines and could be used to accurately detect and image intracellular miRs in an miR-specific manner. Furthermore, the relative levels of intracellular miRs in three different cell lines expressing miR-10b (high, moderate, and low levels) could be monitored using SGNP-MBs. Consequently, these results indicated that SGNP-MBs could have applications as highly potent, efficient nanoprobes to assess intracellular miR levels in living cells.
最近，各种类型的纳米探针被用于通过检测和成像细胞内生物标志物（如 microRNAs(miRs)）来监测活体生物。我们在此提出了一种简单的一锅法来制备具有 miR 检测分子信标的星状金纳米颗粒（SGNP-MBs）；在低 pH 条件下，MBs 能够快速高效地加载到 SGNPs 表面。与传统基于金纳米颗粒的 MBs 相比，SGNPs 能够负载 4.5 倍更高浓度的 MBs，并且表现出 6.4 倍更高的细胞摄取率。我们证明了 SGNP-MBs 能够成功地被内化进入人胃癌细胞系中，并且可以用于以 miR 特异性的方式准确检测和成像细胞内的 miRs。此外，通过 SGNP-MBs 可以监测三种不同表达 miR-10b（高、中、低水平）的细胞系中细胞内 miRs 的相对水平。因此，这些结果表明 SGNP-MBs 可用作高效、有力的纳米探针，用于评估活细胞内的细胞内 miR 水平。
