Huang Xunbing, McNeill Mark Richard, Ma Jingchuan, Qin Xinghu, Tu Xiongbing, Cao Guangchun, Wang Guangjun, Nong Xiangqun, Zhang Zehua
State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing, China.
Scientific Observation and Experimental Station of Pests in Xilin Gol Rangeland, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Xilinhot, Inner Mongolia, China.
J Econ Entomol. 2017 Aug 1;110(4):1831-1840. doi: 10.1093/jee/tox128.
Oedaleus asiaticus B. Bienko is a persistent pest occurring in north Asian grasslands. We found that O. asiaticus feeding on Stipa krylovii Roshev. had higher approximate digestibility (AD), efficiency of conversion of ingested food (ECI), and efficiency of conversion of digested food (ECD), compared with cohorts feeding on Leymus chinensis (Trin.) Tzvel, Artemisia frigida Willd., or Cleistogenes squarrosa (Trin.) Keng. Although this indicated high food utilization efficiency for S. krylovii, the physiological processes and molecular mechanisms underlying these biological observations are not well understood. Transcriptome analysis was used to examine how gene expression levels in O. asiaticus gut are altered by feeding on the four plant species. Nymphs (fifth-instar female) that fed on S. krylovii had the largest variation in gene expression profiles, with a total of 88 genes significantly upregulated compared with those feeding on the other three plants, mainly including nutrition digestive genes of protein, carbohydrate, and lipid digestion. GO and KEGG enrichment also showed that feeding S. krylovii could upregulate the nutrition digestion-related molecular function, biological process, and pathways. These changes in transcripts levels indicate that the physiological processes of activating nutrition digestive enzymes and metabolism pathways can well explain the high food utilization of S. krylovii by O. asiaticus.
亚洲小车蝗（Oedaleus asiaticus B. Bienko）是发生在亚洲北部草原的一种持久性害虫。我们发现，取食克氏针茅（Stipa krylovii Roshev.）的亚洲小车蝗比取食羊草（Leymus chinensis (Trin.) Tzvel）、冷蒿（Artemisia frigida Willd.）或糙隐子草（Cleistogenes squarrosa (Trin.) Keng）的群体具有更高的近似消化率（AD）、摄入食物转化率（ECI）和消化食物转化率（ECD）。尽管这表明亚洲小车蝗对克氏针茅具有较高的食物利用效率，但这些生物学观察结果背后的生理过程和分子机制尚不清楚。转录组分析用于研究取食这四种植物如何改变亚洲小车蝗肠道中的基因表达水平。取食克氏针茅的若虫（五龄雌性）基因表达谱变化最大，与取食其他三种植物的若虫相比，共有88个基因显著上调，主要包括蛋白质、碳水化合物和脂质消化的营养消化基因。GO和KEGG富集分析还表明，取食克氏针茅可上调营养消化相关的分子功能、生物学过程和通路。转录水平的这些变化表明，激活营养消化酶和代谢途径的生理过程可以很好地解释亚洲小车蝗对克氏针茅的高食物利用率。
