Sinadinović J, Savin S, Cvejić D, Mićić J V
INEP, Institute of Endocrinology, Immunology and Nutrition, University of Belgrade, Zemun, Yugoslavia.
Exp Clin Endocrinol. 1988 Sep;92(1):97-105.
We have recently demonstrated that proteolytic activity of lysosomal acid proteases from papillary carcinoma is significantly higher than in morphologically normal thyroid tissue. In the present study the activity of lysosomal acid proteases from parenchymatous proliferated thyroid epithelium, induced by action of antithyroid substances, has been examined in an in vitro system using 125I-labelled rat thyroglobulin as a substrate. Thyroid lysosomes were isolated from rats treated chronically for 3-4 weeks with propylthiouracil (PTU, 0.1% in drinking water) and perchlorate (NaClO4, 200 mg/rat/day) by centrifugation between 800 and 20,000 x g. It was observed that, in contrast to human malignant thyroid tissue, the proteolytic activity of lysosomal acid proteases from antithyroid substance-induced hyperplastic goitre was markedly reduced in comparison with control thyroid tissue (29-50%). Since reduced activity of total lysosomal proteases was found both per unit of wet weight thyroid tissue and per unit of lysosomal proteins, the results suggest that changes in lysosomal enzymes may probably have more quantitative than qualitative nature.
我们最近证实，乳头状癌溶酶体酸性蛋白酶的蛋白水解活性显著高于形态学上正常的甲状腺组织。在本研究中，以125I标记的大鼠甲状腺球蛋白为底物，在体外系统中检测了抗甲状腺物质作用诱导的实质性增生性甲状腺上皮中溶酶体酸性蛋白酶的活性。通过在800至20,000 x g之间离心，从用丙硫氧嘧啶（PTU，饮用水中0.1%）和高氯酸盐（NaClO4，200 mg/大鼠/天）长期处理3 - 4周的大鼠中分离甲状腺溶酶体。观察到，与人类恶性甲状腺组织相反，抗甲状腺物质诱导的增生性甲状腺肿中溶酶体酸性蛋白酶的蛋白水解活性与对照甲状腺组织相比明显降低（29 - 50%）。由于在每单位湿重甲状腺组织和每单位溶酶体蛋白中均发现总溶酶体蛋白酶活性降低，结果表明溶酶体酶的变化可能更多具有数量性质而非质量性质。
