Adeyemo O, Shirai H, Koide S S
Center for Biomedical Research, Population Council, New York, NY 10021.
Gamete Res. 1987 Mar;16(3):251-8. doi: 10.1002/mrd.1120160307.
Cyclic nucleotide levels in the oocytes of the surf clam Spisula solidissima were measured during germinal vesicle breakdown (GVBD) induced by fertilization. The level of cAMP and cGMP in untreated oocytes was 8.23 +/- 0.95 and 4.89 +/- 0.39 pmol/10(6) oocytes. The ratio of cAMP to cGMP ranged from 1.5 to 2.0. The cAMP level in Spisula oocytes fluctuated after fertilization and before GVBD. The cGMP level showed minimal fluctuation, with a tendency to decrease initially followed by a subsequent rise to the basal level in a nonsynchronous manner. These changes were not statistically significant. There was a general increase in protein phosphorylation during the period after fertilization and before GVBD. The greatest increase occurred with proteins of estimated molecular weights of 52, 18, and 12 kD, analyzed by gel electrophoresis and autoradiography.
在受精诱导的生发泡破裂（GVBD）过程中，对 surf 蛤（Spisula solidissima）卵母细胞中的环核苷酸水平进行了测量。未处理的卵母细胞中 cAMP 和 cGMP 的水平分别为 8.23±0.95 和 4.89±0.39 pmol/10⁶ 个卵母细胞。cAMP 与 cGMP 的比值在 1.5 至 2.0 之间。受精后至 GVBD 前，Spisula 卵母细胞中的 cAMP 水平波动。cGMP 水平波动最小，最初有下降趋势，随后以非同步方式回升至基础水平。这些变化无统计学意义。受精后至 GVBD 前，蛋白质磷酸化普遍增加。通过凝胶电泳和放射自显影分析，估计分子量为 52、18 和 12 kD 的蛋白质增加最为显著。
