Wu Renhong, Citovsky Vitaly
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, United States.
Biochem Biophys Res Commun. 2017 Jul 8;488(4):609-613. doi: 10.1016/j.bbrc.2017.05.085. Epub 2017 May 17.
Understanding how root hair development is controlled is important for understanding of many fundamental aspects of plant biology. Previously, we identified two plant-specific adaptor proteins GIR1 and GIR2 that interact with the major regulator of root hair development GL2 and suppress formation of root hair. Here, we show that GIR1 and GIR2 also interact with the co-repressor TOPLESS (TPL). This interaction required the GIR1 protein EAR motif, and was essential for the transcriptional repressor activity of GIR1. Both GIR1 and GIR2 promoted histone hypoacetylation of their target chromatin. Potentially, GIR1 and GIR2 might may link TPL to and participate in epigenetic regulation of root hair development.
了解根毛发育是如何被调控的，对于理解植物生物学的许多基本方面至关重要。此前，我们鉴定出了两种植物特异性衔接蛋白GIR1和GIR2，它们与根毛发育的主要调节因子GL2相互作用，并抑制根毛的形成。在此，我们表明GIR1和GIR2也与共抑制因子TOPLESS（TPL）相互作用。这种相互作用需要GIR1蛋白的EAR基序，并且对于GIR1的转录抑制活性至关重要。GIR1和GIR2都促进了其靶染色质的组蛋白低乙酰化。GIR1和GIR2可能将TPL与根毛发育的表观遗传调控联系起来并参与其中。
