Sienkiewicz T, Mohamed S H, Lipke B
Humboldt-Universität zu Berlin, Sektion Nahrungsgüterwirtschaft und Lebensmitteltechnologie, DDR.
Nahrung. 1988;32(8):749-54. doi: 10.1002/food.19880320810.
Ion exchange-chromatography method with the use of hydroxy apatite is considered one of the methods used for the casein fractionation particularly those containing phosphate in its composition. Simplicity of hydroxyapatite-preparation and its easily use in casein fractionation in batches are advantages of this method. Sephadex G-25 or Agarose is used to easement the sample passage through the column. By using of hydroxy-apatite in batches, alpha s-casein can easily separated with the use of buffer solution 0.006 M phosphate at pH 6.6 and the elution is carried out by buffer solution containing 0.25 M KH2PO4, 0.2 M KCl, 4.5 M urea and 0.002 M di-thiotreitol. Before the elution of alpha s-casein, beta- and kappa-casein are separated using buffer solutions containing 0.005 M and 0.1 M KH2PO4 in addition to the previously mentioned components.
使用羟基磷灰石的离子交换色谱法被认为是用于酪蛋白分级分离的方法之一，特别是那些在其组成中含有磷酸盐的酪蛋白。羟基磷灰石制备简单且易于批量用于酪蛋白分级分离是该方法的优点。葡聚糖凝胶G-25或琼脂糖用于使样品更顺畅地通过柱子。通过批量使用羟基磷灰石，在pH 6.6的0.006 M磷酸盐缓冲溶液中可轻松分离出αs-酪蛋白，并用含有0.25 M KH2PO4、0.2 M KCl、4.5 M尿素和0.002 M二硫苏糖醇的缓冲溶液进行洗脱。在洗脱αs-酪蛋白之前，除了上述成分外，使用含有0.005 M和0.1 M KH2PO4的缓冲溶液分离β-和κ-酪蛋白。
