纳米颗粒功能化及β-淀粉样蛋白残基（32-37）多重甲基化对革兰氏阳性菌的影响。
Effect of GNP functionalisation and multiple -methylation of -amyloid residue (32-37) on Gram-positive bacterium.
作者信息
Hemmaragala Nanjundaswamy Marishetty, Abrahamse Heidi, George Blassan P
机构信息
Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, South Africa.
出版信息
IET Nanobiotechnol. 2017 Jun;11(4):377-382. doi: 10.1049/iet-nbt.2016.0083.
In the previous report, the authors showed the gold nanoparticle (GNP) functionalised multiple -methylated fragments of the residue (32-37) of beta ()-amyloid protein (1-42), CGGIGLMVG and CGGGGGIGLMVG toward disruption of -amyloid (1-42), the predominant component of senile plaques. Herein the in vitro antimicrobial activities of both normal and multiple -methylated sequences of CGGIGLMVG and CGGGGGIGLMVG were screened and it was found that all the eight sequences including four (non-functionalised with GNP) to possess activity against both Gram-positive [ (ATCC 43300) and Enterococcus faecalis (ATCC 5129)] and Gram-negative [Escherichia coli (ATCC 35218), (ATCC 27853) and (ATCC 700603)] bacteria. Among them, -methylated sequences CGGIGLMVG and CGGGGGIGLMVG shown remarkable activity against Gram-positive bacteria.
在之前的报告中，作者展示了用金纳米颗粒（GNP）功能化的β-淀粉样蛋白（1-42）残基（32-37）的多个甲基化片段，即CGGIGLMVG和CGGGGGIGLMVG，它们可破坏老年斑的主要成分β-淀粉样蛋白（1-42）。在此，对CGGIGLMVG和CGGGGGIGLMVG的正常序列和多个甲基化序列的体外抗菌活性进行了筛选，发现所有八个序列，包括四个（未用GNP功能化），对革兰氏阳性菌[金黄色葡萄球菌（ATCC 43300）和粪肠球菌（ATCC 5129）]和革兰氏阴性菌[大肠杆菌（ATCC 35218）、铜绿假单胞菌（ATCC 27853）和鲍曼不动杆菌（ATCC 700603）]均具有活性。其中，甲基化序列CGGIGLMVG和CGGGGGIGLMVG对革兰氏阳性菌表现出显著活性。
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