Paccotti P, Terzolo M, Piovesan A, Torta M, Vignani A, Angeli A
Dipartimento di Biomedicina, Università degli Studi di Torino, Ospedale S. Luigi, Orbassano, Italy.
Chronobiologia. 1988 Oct-Dec;15(4):279-87.
We evaluated the effect of an acute oral administration of 2 dosages (100 and 1 mg) of melatonin (MT) vs placebo (PL) on pituitary release of LH, FSH, TSH and PRL after GnRH + TRH and on the adrenocortical release of cortisol, aldosterone and progesterone after ACTH in healthy adult males. We carried out a double blind study on 6 volunteers in winter-early spring, at 2 opposite phases of the circadian cycle: 08(00) and 20(00). Injection of GnRH (100 micrograms), TRH (200 micrograms) and ACTH (10 micrograms of the synthetic analogue ACTH 1-17, alsactide) was performed 1 h after MT or PL ingestion. The measurement of plasma MT levels confirmed its effective gastrointestinal absorption after both doses. The hormonal patterns were superimposable after MT and PL. A higher response of FSH, PRL, cortisol and aldosterone was observed in the evening vs morning protocols independently of previous treatment (MT or PL). Our data demonstrate that the acute oral administration of 2 different doses of MT at 2 opposite circadian stages is ineffective as to the modification of a variety of pituitary and adrenocortical responses in human male subjects. The circadian chronosusceptibility of pituitary and adrenocortical cells to specific stimuli deserves interest to future investigation.
我们评估了在健康成年男性中，急性口服两种剂量（100毫克和1毫克）的褪黑素（MT）与安慰剂（PL），对促性腺激素释放激素（GnRH）加促甲状腺激素释放激素（TRH）后垂体释放促黄体生成素（LH）、促卵泡生成素（FSH）、促甲状腺激素（TSH）和催乳素（PRL）的影响，以及对促肾上腺皮质激素（ACTH）后肾上腺皮质释放皮质醇、醛固酮和孕酮的影响。我们在冬季至早春对6名志愿者进行了一项双盲研究，在昼夜节律周期的两个相反阶段：08(00)和20(00)。在摄入MT或PL后1小时注射GnRH（100微克）、TRH（200微克）和ACTH（10微克合成类似物ACTH 1-17，即阿尔沙肽）。血浆MT水平的测量证实了两种剂量后其在胃肠道的有效吸收。MT和PL后的激素模式是可叠加的。与之前的治疗（MT或PL）无关，在晚上的方案中观察到FSH、PRL、皮质醇和醛固酮的反应高于早晨的方案。我们的数据表明，在昼夜节律的两个相反阶段急性口服两种不同剂量的MT，对改变男性受试者各种垂体和肾上腺皮质反应是无效的。垂体和肾上腺皮质细胞对特定刺激的昼夜节律易感性值得未来研究关注。
