Biomolecular Engineering Laboratory, Department of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, Iran.
Biomolecular Engineering Laboratory, Department of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, Iran.
Food Chem. 2017 Oct 15;233:282-289. doi: 10.1016/j.foodchem.2017.04.126. Epub 2017 Apr 21.
Nature-made inherent transporting property of beta-lactoglobulin (BLG) was exploited to develop delivery systems for quercetin. After binding to BLG, quercetin was nanoencapsulated within soft-condensed nanostructures of BLG and sodium alginate (ALG). Fluorimetry results revealed that quercetin could bind to BLG even at acidic conditions. The amounts of stoichiometry binding (n) were 1.24 and 1.62 at pH values of 4 and 7, respectively. Formation of core-shell type nanostructures was confirmed by transmission electron microscopy. Quercetin was efficiently entrapped (>93%). The ejection from the carrier was very limited over time (<1% during 1month). The protection of nanoencapsulated quercetin was at least 3 times better than that of free quercetin. Quercetin was not released (<3.5% during 6h) in simulated gastric fluids (pH 1.2 and 4); while, a sustained release (77% during 12h) was observed in simulated intestinal fluid (pH 7.4).
利用β-乳球蛋白（BLG）的天然内在输送特性，开发了用于槲皮素的递送系统。与 BLG 结合后，槲皮素被纳米封装在 BLG 和海藻酸钠（ALG）的软凝聚纳米结构内。荧光法结果表明，即使在酸性条件下，槲皮素也可以与 BLG 结合。在 pH 值为 4 和 7 时，结合的化学计量数（n）分别为 1.24 和 1.62。透射电子显微镜证实形成了核壳型纳米结构。槲皮素的包封效率很高（>93%）。随着时间的推移，从载体中的释放非常有限（1 个月内<1%）。纳米封装的槲皮素的保护作用至少比游离槲皮素好 3 倍。在模拟胃液（pH 1.2 和 4）中，槲皮素没有释放（6 小时内<3.5%）；然而，在模拟肠液（pH 7.4）中观察到持续释放（12 小时内 77%）。
