Straubinger B, Thiebe R, Pech M, Zachau H G
Institut für Physiologische Chemie, Universität München, F.R.G.
Gene. 1988 Sep 30;69(2):209-14. doi: 10.1016/0378-1119(88)90431-3.
A group of highly homologous transposed human V kappa I genes, which we call the Z family, was characterized. To date four members, ZI-ZIV, comprising about 230 kb, have been analyzed on cosmid clones. The largest region (ZI) has a length of 85 kb. The Z regions show extensive homology to each other according to restriction maps and hybridization data. In each Z region a solitary V kappa I gene was found. No V kappa genes of other subgroups were detected by hybridization. The nucleotide sequence of the ZI gene revealed a non-processed V kappa I pseudogene. Hybridization experiments with DNAs from rodent/human cell hybrids and other experimental data indicate that some and possibly all members of the Z family lie outside of the kappa locus which is located on chromosome 2; they have been transposed to other chromosomes. Because of their separation from the J kappa C kappa gene segment, the Z genes can be classified as pseudogenes independent of their sequences. We postulate that the Z family arose by amplification event(s). The Z regions can also be regarded as a small family of very long repetitive sequences.
我们鉴定了一组高度同源的转座人VκI基因，我们将其称为Z家族。迄今为止，已在黏粒克隆上分析了由大约230 kb组成的四个成员ZI - ZIV。最大的区域（ZI）长度为85 kb。根据限制性图谱和杂交数据，Z区域彼此显示出广泛的同源性。在每个Z区域中发现了一个单独的VκI基因。通过杂交未检测到其他亚组的Vκ基因。ZI基因的核苷酸序列揭示了一个未加工的VκI假基因。用啮齿动物/人类细胞杂种的DNA进行的杂交实验和其他实验数据表明，Z家族的一些成员甚至可能所有成员都位于位于2号染色体上的κ基因座之外；它们已转座到其他染色体上。由于它们与JκCκ基因片段分离，因此Z基因可被归类为假基因，而与其序列无关。我们推测Z家族是由扩增事件产生的。Z区域也可被视为一个非常长的重复序列的小家族。
