Chiu Yi-Ting, Chen Yi-Hsuan, Wang Ting-Shaun, Yen Hung-Kai, Lin Tsair-Fuh
Department of Environmental Engineering, National Cheng Kung University, Tainan 70101, Taiwan.
Department of Biological Science and Technology, Meiho University, Pingtung 91202, Taiwan.
Int J Environ Res Public Health. 2017 May 20;14(5):547. doi: 10.3390/ijerph14050547.
Harmful cyanobacteria have been an important concern for drinking water quality for quite some time, as they may produce cyanotoxins and odorants. and are two common harmful cyanobacterial genera detected in freshwater lakes and reservoirs, with microcystins (MCs) and cylindrospermopsin (CYN) as their important metabolites, respectively. In this study, two sets of duplex qPCR systems were developed, one for quantifying potentially-toxigenic and , and the other one for cylindrospermopsin-producing cyanobacteria and . The duplex qPCR systems were developed and validated in the laboratory by using 338 samples collected from 29 reservoirs in Taiwan and her offshore islands. Results show that cell numbers of and enumerated with microscopy, and MCs and CYN concentrations measured with the enzyme-linked immuno-sorbent assay method, correlated well with their corresponding gene copies determined with the qPCR systems (range of coefficients of determination R² = 0.392-0.740). The developed qPCR approach may serve as a useful tool for the water industry to diagnose the presence of harmful cyanobacteria and the potential presence of cyanotoxins in source waters.
有害蓝藻在相当长一段时间内一直是饮用水水质的重要关注点，因为它们可能产生蓝藻毒素和气味物质。 和 是在淡水湖泊和水库中检测到的两种常见有害蓝藻属，分别以微囊藻毒素（MCs）和柱孢藻毒素（CYN）作为其重要代谢产物。在本研究中，开发了两套双重定量聚合酶链反应（qPCR）系统，一套用于定量潜在产毒的 和 ，另一套用于定量产柱孢藻毒素的蓝藻和 。双重qPCR系统在实验室中通过使用从台湾及其离岛的29个水库采集的338个样本进行了开发和验证。结果表明，通过显微镜计数的 和 的细胞数量，以及用酶联免疫吸附测定法测量的MCs和CYN浓度，与通过qPCR系统测定的相应基因拷贝数具有良好的相关性（决定系数R²范围 = 0.392 - 0.740）。所开发的qPCR方法可作为水行业诊断源水中有害蓝藻的存在以及蓝藻毒素潜在存在的有用工具。
