Sato E, Kawamura N, Ishibashi T
Department of Animal Science, Faculty of Agriculture, Kyoto University, Japan.
J Dairy Sci. 1988 Dec;71(12):3482-8. doi: 10.3168/jds.S0022-0302(88)79954-3.
The effects of the dibutyryl cyclic adenosine 3',5'-monophosphate, calmodulin antagonist W7, and ovarian glycosaminoglycans on polar body extrusion, "nucleus" formation, and segmentation of bovine oocytes were examined in vitro. Dibutyryl cyclic adenosine 3',5'-monophosphate (100 microM) accelerated the extrusion of first polar body; however, W7 (20 microM) suppressed the formation of the first polar body. Spontaneous "nucleus" formation resembling pronucleus formation in oocytes was accelerated in the presence of dibutyryl cyclic adenosine 3',5'-monophosphate at concentrations of greater than 20 microM. The spontaneous segmentation, which is a degenerative change, was suppressed in the presence of bovine follicular fluid-glycosaminoglycans at concentrations of 250 micrograms/ml or greater. These results may indicate that maturation, activation, and degeneration of bovine oocytes in culture are modulated by dibutyryl cyclic adenosine 3',5'-monophosphate, W7, and ovarian glycosaminoglycans.
在体外研究了二丁酰环磷腺苷3',5'-单磷酸、钙调蛋白拮抗剂W7和卵巢糖胺聚糖对牛卵母细胞极体排出、“核”形成及卵裂的影响。二丁酰环磷腺苷3',5'-单磷酸（100微摩尔）加速了第一极体的排出；然而，W7（20微摩尔）抑制了第一极体的形成。在浓度大于20微摩尔的二丁酰环磷腺苷3',5'-单磷酸存在下，卵母细胞中类似原核形成的自发“核”形成加速。浓度为250微克/毫升或更高的牛卵泡液-糖胺聚糖可抑制作为退化性变化的自发卵裂。这些结果可能表明，培养中牛卵母细胞的成熟、激活和退化受二丁酰环磷腺苷3',5'-单磷酸、W7和卵巢糖胺聚糖的调节。
