a Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University , Guangzhou , China.
b Department of Biomedical Engineering , Jinan University , Guangzhou , China.
Artif Cells Nanomed Biotechnol. 2018 May;46(3):544-550. doi: 10.1080/21691401.2017.1328687. Epub 2017 May 22.
When hydrophobically modified chitosan (HM-CHI) comes into contact with red blood cells (RBCs), it can cause agglomeration. This property leads HM-CHI to be a potential agent to form a rapid haemostatic plug to stop bleeding at wound sites. In this study, we investigated the properties of the HM-CHI biopolymer to act as an agent that rapidly clots blood. We have examined the synthesis and structural characteristics of HM-CHI - blood substances using X-ray diffraction, infrared spectroscopy and elemental analysis. To understand the rheological behaviour of RBCs within HM-CHI, we have studied the effects of HM-CHI on RBC aggregation and morphology using a rheometer and scanning electron microscopy (SEM), respectively. When mixed with sodium citrate, HM-CHI rapidly transformed human blood into an elastic gel. In contrast, un-modified chitosan (i.e. without hydrophobes) was unable to clot blood. The hydrophobias within HM-CHI entered into the RBC membranes and connected to the cells by a sample-spanning network, which subsequently led to the formation of an elastic gel. The gelling ability of HM-CHI is similar to that of fibrin-based sealants, but at a much lower cost and greater availability.
当疏水性改性壳聚糖（HM-CHI）与红细胞（RBC）接触时，它会引起聚集。这种特性使 HM-CHI 成为一种潜在的试剂，可在伤口部位形成快速止血塞以止血。在这项研究中，我们研究了 HM-CHI 生物聚合物作为一种快速凝血试剂的特性。我们使用 X 射线衍射、红外光谱和元素分析研究了 HM-CHI-血液物质的合成和结构特征。为了了解 HM-CHI 内 RBC 的流变行为，我们使用流变仪和扫描电子显微镜（SEM）分别研究了 HM-CHI 对 RBC 聚集和形态的影响。当与柠檬酸钠混合时，HM-CHI 可使人类血液迅速转化为弹性凝胶。相比之下，未改性的壳聚糖（即不含疏水性基团）无法凝血。HM-CHI 中的疏水性基团进入 RBC 膜，并通过横跨样品的网络与细胞相连，从而形成弹性凝胶。HM-CHI 的胶凝能力类似于基于纤维蛋白的密封剂，但成本更低、可用性更高。
