Djeraba Zineb, Benlabidi Fatiha, Djaballah-Ider Fatma Zohra, Medjeber Oussama, Arroul-Lammali Amina, Belguendouz Houda, Otmani Fifi, Touil-Boukoffa Chafia
a Cytokines and NO Synthases Team, Laboratory of Cellular and Molecular Biology (LBCM), Biological Sciences Faculty , University of Sciences and Technology Houari Boumediene (USTHB) , Algiers , Algeria.
b Internal Medicine Service at Mustapha Bacha Hospital , Algiers , Algeria.
Immunopharmacol Immunotoxicol. 2017 Aug;39(4):243-250. doi: 10.1080/08923973.2017.1327967. Epub 2017 May 22.
Behçet's disease (BD) is an inflammatory multisystemic disorder associated with orogenital ulcers, uveitis and skin lesions with unpredictable episodes of exacerbations and remissions. Even though several immunological and environmental factors contribute to BD progression, its ethiopathogenesis remains uncertain and elusive. Considered as one of the potent environmental factors that can increase prevalence of some autoimmune and inflammatory disorders, vitamin D deficiency has been linked to several diseases as BD. The aim of this study is to assess vitamin D status in Algerian BD patients and its relationship with disease activity. Immunomodulatory effect of this vitamin on nitric oxide (NO), inflammatory mediator, was also undertaken. Serum 25(OH) vitamin D levels were measured in healthy controls (HC), active and inactive BD patients with an electrochemiluminescence method. After treatment of HCs' and patients' peripheral blood mononuclear cells with different concentrations of vitamin D3, NO production was evaluated with Griess method, while inducible nitric oxide synthase (iNOS) and NF-κB expression with immunofluorescence test. A high decrease of vitamin D levels was noted in active BD patients compared to those of inactive stage and HC. However, a higher NO production was observed during active stage of BD compared to inactive one. In inactive BD, vitamin D levels correlates negatively with NO. Interestingly, vitamin D3 inhibits ex vivo NO production, iNOS and NF-κB expression in BD patients. In conclusion, vitamin D deficiency was associated with active BD. This vitamin down-modulates NO production in BD patients, suggesting that it may be considered as promising therapy modulating inflammation during BD.
白塞病（BD）是一种炎症性多系统疾病，伴有口生殖器溃疡、葡萄膜炎和皮肤病变，病情发作和缓解难以预测。尽管多种免疫和环境因素促使BD进展，但其发病机制仍不明确且难以捉摸。维生素D缺乏被认为是可增加某些自身免疫性和炎症性疾病患病率的重要环境因素之一，它与包括BD在内的多种疾病有关。本研究旨在评估阿尔及利亚BD患者的维生素D状况及其与疾病活动度的关系。还研究了这种维生素对炎症介质一氧化氮（NO）的免疫调节作用。采用电化学发光法测定健康对照者（HC）、活动期和非活动期BD患者的血清25(OH)维生素D水平。用不同浓度的维生素D3处理HC和患者的外周血单核细胞后，采用格里斯法评估NO生成，同时通过免疫荧光试验检测诱导型一氧化氮合酶（iNOS）和核因子κB（NF-κB）的表达。与非活动期BD患者和HC相比，活动期BD患者的维生素D水平显著降低。然而，与非活动期相比，BD活动期观察到更高的NO生成。在非活动期BD中，维生素D水平与NO呈负相关。有趣的是，维生素D3可抑制BD患者体外NO生成、iNOS和NF-κB表达。总之，维生素D缺乏与活动期BD相关。这种维生素可下调BD患者的NO生成，提示其可能是BD炎症调节的一种有前景的治疗方法。
