a Department of Plant Pathology , Citrus Research and Education Center, IFAS, University of Florida , Lake Alfred , FL , USA.
Plant Signal Behav. 2017 Jun 3;12(6):e1327497. doi: 10.1080/15592324.2017.1327497. Epub 2017 May 22.
Candidatus Liberibacter asiaticus is a phloem restricted bacterium that causes citrus greening disease or huanglongbing (HLB), a major treat to commercial citrus production in Florida. It is transmitted by the Asian citrus psyllid, a phloem sap-feeding insect. Studies conducted on the composition of citrus phloem sap revealed the presence amino acids, organic acids and sugars and of low amounts of free fatty acids. In the present study, the phloem sap of 12 citrus varieties with different degrees of tolerance to HLB were extracted with ethyl acetate and analyzed by GC-MS after derivatization with boron trifluoride, a fatty acid-specific reagent. Nine free fatty acids were detected in all varieties. Of the 9 fatty acids detected, only capric acid was significantly different among varieties.
亚洲韧皮杆菌是一种局限于韧皮部的细菌，会引起柑橘黄龙病（HLB），这是佛罗里达州商业柑橘生产的主要威胁。它通过亚洲柑橘木虱传播，这是一种吸食韧皮部汁液的昆虫。对柑橘韧皮部汁液成分的研究表明，存在氨基酸、有机酸和糖，以及少量游离脂肪酸。在本研究中，用乙酸乙酯提取了 12 个对 HLB 具有不同耐受程度的柑橘品种的韧皮部汁液，并用三氟化硼衍生化后用 GC-MS 进行分析，三氟化硼是一种特定于脂肪酸的试剂。在所有品种中都检测到了 9 种游离脂肪酸。在所检测到的 9 种脂肪酸中，只有癸酸在品种间存在显著差异。
