Altamura Louis A, Cazares Lisa H, Coyne Susan R, Jaissle James G, Jespersen Alyssa M, Ahmed Sundus, Wasieloski Leonard P, Garrison Jeff, Kulesh David A, Brueggemann Ernst E, Kenny Tara, Ward Michael D, Harbourt David E, Minogue Timothy D
Diagnostic Systems Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, United States.
Molecular and Translational Sciences Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, United States; DOD Biotechnology High Performance Computing Software Applications Institute, Telemedicine and Advanced Technology Research Center, US Army Medical Research and Materiel Command, Fort Detrick, MD 21702, United States.
J Virol Methods. 2017 Oct;248:1-6. doi: 10.1016/j.jviromet.2017.05.010. Epub 2017 May 19.
Research involving biosafety level 3 pathogens such as West Nile virus (WNV) is often limited by the limited space and technical constraints of these environments. To conduct complex analytical studies outside of high containment, robust and reliable inactivation methods are needed that maintain compatibility with downstream assays. Here we report the inactivation of WNV in spiked serum samples using a commercially available SDS-PAGE sample buffer for proteomic studies. Using this method, we demonstrate its utility by identification proteins differentially expressed in the serum of mice experimentally infected with WNV.
涉及生物安全3级病原体（如西尼罗河病毒，WNV）的研究常常受到这些环境空间有限和技术限制的制约。为了在高防护设施之外进行复杂的分析研究，需要强大且可靠的灭活方法，且该方法要与下游检测兼容。在此，我们报告了使用一种用于蛋白质组学研究的市售SDS-PAGE样品缓冲液对加标血清样本中的WNV进行灭活。通过这种方法，我们通过鉴定在实验感染WNV的小鼠血清中差异表达的蛋白质，证明了其效用。
