Bao Haibo, Zhang Yixi, Liu Yang, Yu Na, Liu Zewen
Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China.
Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China.
Neurochem Int. 2017 Sep;108:381-387. doi: 10.1016/j.neuint.2017.05.015. Epub 2017 May 19.
Failures in expressing insect nAChRs (nicotinic acetylcholine receptors) limit the understanding of their functional properties, although insect nAChRs have been developed as insecticide targets for decades. Some accessory proteins can modulate nAChRs, but only a few have been identified in insects, including lynx from Ly-6/neurotoxin superfamily. Until now, only one to three lynx were identified in one insect species. Here, 11 lynx were identified in Locusta migratoria with common features of Ly-6/neurotoxin members. The effects of these lynx on seven hybrid nAChRs functionally constructed in Xenopus oocytes were tested, in which each nAChR subtype contained one L. migratoria α subunit plus rat β2 subunit. One lynx could selectively modulate several nAChR subtypes to significantly increase the amplitude of epibatidine-evoked currents, while Loc-lynx10 acted on all test nAChRs. Among nine lynx with obvious modulation effects to increase inward currents, only four significantly increased the specific [H]epibatidine binding, and the binding increase existed in all modulated nAChRs by these lynx. The results indicated that the modulation mode was decided by the specific lynx and had nothing to do with nAChR subtypes. This study provides an approximate panorama for the functional interaction net between L. migratoria lynx and nAChRs, although some other nAChRs need further tests.
尽管几十年来昆虫烟碱型乙酰胆碱受体（nAChRs）已被开发为杀虫剂靶标，但在表达这些受体方面的失败限制了对其功能特性的了解。一些辅助蛋白可以调节nAChRs，但在昆虫中仅鉴定出少数几种，包括来自Ly-6/神经毒素超家族的lynx。到目前为止，在一种昆虫物种中仅鉴定出一到三种lynx。在此，在飞蝗中鉴定出11种lynx，它们具有Ly-6/神经毒素成员的共同特征。测试了这些lynx对在非洲爪蟾卵母细胞中功能性构建的七种杂交nAChRs的影响，其中每种nAChR亚型包含一个飞蝗α亚基和大鼠β2亚基。一种lynx可以选择性地调节几种nAChR亚型，以显著增加埃博霉素诱发电流的幅度，而Loc-lynx10作用于所有测试的nAChRs。在九种具有明显调节作用以增加内向电流的lynx中，只有四种显著增加了特异性[H]埃博霉素结合，并且这些lynx在所有调节的nAChRs中均存在结合增加。结果表明，调节模式由特定的lynx决定，与nAChR亚型无关。本研究提供了一个关于飞蝗lynx与nAChRs之间功能相互作用网络的大致全景，尽管一些其他nAChRs需要进一步测试。
