Shaliutina Olena, Shaliutina-Kolešová Anna, Lebeda Ievgen, Rodina Marek, Gazo Ievgeniia
University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.
University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.
Toxicol In Vitro. 2017 Sep;43:9-15. doi: 10.1016/j.tiv.2017.05.006. Epub 2017 May 19.
The sturgeon is a highly endangered fish mostly due to over-fishing, habitat destruction, and water pollution. Nonylphenol (NP), propranolol (PN), and diethylstilbestrol (DES) are multifunctional xenobiotic compounds used in a variety of commercial and industrial products. The mechanism by which these xenobiotic compounds interfere with fish reproduction is not fully elucidated. This study assessed the effect of NP, PN, and DES on motility parameters, membrane integrity, and oxidative/antioxidant status in sterlet Acispenser ruthenus spermatozoa. Spermatozoa were incubated with several concentrations of target substances for 1h. Motility rate and velocity of spermatozoa decreased in the presence of xenobiotics in a dose-dependent manner compared with controls. A significant decrease in membrane integrity was recorded with exposure to 5μM of NP, 25μM of PN, and 50μM of DES. After 1h exposure at higher tested concentrations NP (5-25μM), PN (25-100μM), and DES (50-200μM), oxidative stress was apparent, as reflected by significantly higher levels of protein and lipid oxidation and significantly greater superoxide dismutase activity. The results demonstrated that NP, PN, and DES can induce reactive oxygen species stress in fish spermatozoa, which could impair sperm quality and the antioxidant defence system and decrease the percentage of intact sperm cells.
鲟鱼是一种高度濒危的鱼类，主要原因是过度捕捞、栖息地破坏和水污染。壬基酚（NP）、普萘洛尔（PN）和己烯雌酚（DES）是用于各种商业和工业产品的多功能外源性化合物。这些外源性化合物干扰鱼类繁殖的机制尚未完全阐明。本研究评估了NP、PN和DES对俄罗斯鲟精子活力参数、膜完整性以及氧化/抗氧化状态的影响。精子与几种浓度的目标物质孵育1小时。与对照组相比，在外源性物质存在的情况下，精子的活力率和速度呈剂量依赖性下降。暴露于5μM的NP、25μM的PN和50μM的DES时，膜完整性显著下降。在较高测试浓度NP（5-25μM）、PN（25-100μM）和DES（50-200μM）下暴露1小时后，氧化应激明显，表现为蛋白质和脂质氧化水平显著升高以及超氧化物歧化酶活性显著增强。结果表明，NP、PN和DES可诱导鱼类精子中的活性氧应激，这可能损害精子质量和抗氧化防御系统，并降低完整精子细胞的百分比。
