响应调节基因 slr1037 和 sll0039 的共过表达提高了集胞藻 PCC 6803 对 1-丁醇的耐受性。
Co-overexpression of response regulator genes slr1037 and sll0039 improves tolerance of Synechocystis sp. PCC 6803 to 1-butanol.
机构信息
Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, PR China; Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, PR China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, PR China.
Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, PR China; Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, PR China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, PR China.
出版信息
Bioresour Technol. 2017 Dec;245(Pt B):1476-1483. doi: 10.1016/j.biortech.2017.04.112. Epub 2017 May 4.
In this study, two response regulator (RR) encoding genes slr1037 as well as sll0039 were co-overexpressed in Synechocystis sp. PCC 6803 by metabolic engineering and the 1-butanol tolerance was successfully improved by 133%. Aiming to explore the possible mechanisms for the enhancing 1-butanol tolerance, a quantitative iTRAQ-LC-MS/MS proteomics approach was then employed, identifying 216 up-regulated and 99 down-regulated proteins compared to wild type after 1-butanol treatment. This study mapped the potential target genes regulated by Slr1037 and Sll0039 and demonstrated the feasibility of engineering response regulators for modifying the biofuel tolerance in cyanobacteria.
在这项研究中，通过代谢工程共过表达了两个应答调节子（RR）编码基因 slr1037 和 sll0039，使集胞藻 PCC 6803 的 1-丁醇耐受性成功提高了 133%。为了探索提高 1-丁醇耐受性的可能机制，然后采用了定量 iTRAQ-LC-MS/MS 蛋白质组学方法，与野生型相比，1-丁醇处理后鉴定出 216 个上调和 99 个下调的蛋白质。本研究绘制了 Slr1037 和 Sll0039 调控的潜在靶基因图谱，并证明了工程应答调节子修饰蓝藻生物燃料耐受性的可行性。
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