脂肪酸和溶血磷脂对钙依赖性中性蛋白酶活性的调节作用。

Modulation of calcium-dependent neutral protease activity by fatty acids and lysophospholipids.

作者信息

Zalewska T, Strosznajder J, Kawashima S

机构信息

Department of Neurochemistry, Medical Research Centre, Polish Academy of Sciences, Warsaw.

出版信息

Neurochem Pathol. 1988 Apr;8(2):79-89. doi: 10.1007/BF03160137.

DOI:10.1007/BF03160137

PMID:2853315

Abstract

The effect of fatty acids and lysophospholipids on calcium-activated neutral protease (CANP) was investigated. mu CANP, low calcium ion (microM concentration)-requiring CANP is more strongly inhibited by unsaturated fatty acids than is mCANP--the high calcium ion (mM concentration)-requiring form. Lysophospholipids in concentrations ranging from 10(-5) M to 10(-3) M inhibit mu CANP exclusively, whereas mCANP activity is unaffected or even slightly increased. Calpastatin decreases the activity of mCANP and, in the presence of polyunsaturated fatty acids such as docosahexaenoic acid, the inhibition is not increased. In the presence of lysophosphatidyl-ethanolamine, however, the inhibition of mCANP by calpastatin does not occur. The results indicate that fatty acids and lysocompounds liberated under different physiological and pathological conditions may modulate calcium-activated neutral protease.

摘要

研究了脂肪酸和溶血磷脂对钙激活中性蛋白酶（CANP）的影响。μCANP是需要低钙离子（微摩尔浓度）的CANP，与需要高钙离子（毫摩尔浓度）的形式mCANP相比，不饱和脂肪酸对其抑制作用更强。浓度范围为10^(-5) M至10^(-3) M的溶血磷脂仅抑制μCANP，而mCANP活性不受影响甚至略有增加。钙蛋白酶抑制蛋白降低mCANP的活性，并且在存在多不饱和脂肪酸如二十二碳六烯酸的情况下，抑制作用不会增强。然而，在存在溶血磷脂酰乙醇胺的情况下，钙蛋白酶抑制蛋白对mCANP的抑制作用不会发生。结果表明，在不同生理和病理条件下释放的脂肪酸和溶血化合物可能调节钙激活中性蛋白酶。