前列腺素作为还原剂：腺苷酸环化酶激活模型？

Prostaglandins as reducing agents: a model of adenylate cyclase activation?

作者信息

Peterson D A, Kelly B, Mehta N, Gerrard J M

机构信息

Research Service, V. A. Medical Center, Minneapolis, MN 55417.

出版信息

Prostaglandins. 1988 Nov;36(5):667-71. doi: 10.1016/0090-6980(88)90012-3.

DOI:10.1016/0090-6980(88)90012-3

PMID:2853423

Abstract

It has been suggested that adenylate cyclase activation involves reduction of a disulfide linkage. Prostaglandin E1 (PGE1), prostaglandin E2 (PGE2), prostaglandin I2 (PGI2) and prostaglandin F2 alpha (PGF2 alpha) were tested for their ability to act as reducing agents with either cytochrome c, or the disulfide 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB), the latter with a catalytic amount of ferric chloride. PGE1, PGE2, and PGI2 significantly reduced cytochrome c while PGF2 alpha did not. PGE1, PGE2 and PGI2 reduced DTNB while PGF2 alpha did not. The results are consistent with the postulate that prostaglandins which are effective in activating adenylate cyclase can act as reducing agents and might be involved in reductive activation of adenylate cyclase.

摘要

有人提出，腺苷酸环化酶的激活涉及二硫键的还原。测试了前列腺素E1（PGE1）、前列腺素E2（PGE2）、前列腺素I2（PGI2）和前列腺素F2α（PGF2α）作为还原剂与细胞色素c或二硫键5,5'-二硫代双（2-硝基苯甲酸）（DTNB）反应的能力，后者加入催化量的氯化铁。PGE1、PGE2和PGI2能显著还原细胞色素c，而PGF2α则不能。PGE1、PGE2和PGI2能还原DTNB，而PGF2α则不能。这些结果与以下假设一致：能有效激活腺苷酸环化酶的前列腺素可作为还原剂，并可能参与腺苷酸环化酶的还原激活。