Chaudhury M F, Zhu J J, Skoda S R
USDA-ARS, 109 C Entomology Hall, University of Nebraska-Lincoln, Lincoln, NE 68583.
USDA-ARS, 305 Entomology Hall, University of Nebraska-Lincoln, Lincoln, NE 68583.
J Econ Entomol. 2017 Aug 1;110(4):1929-1934. doi: 10.1093/jee/tox145.
Volatile chemicals from waste artificial larval media as well as from bovine blood inoculated with bacteria isolated from screwworm-infested wounds attract gravid females of Cochliomyia hominivorax Coquerel and Cochliomyia macellaria (F.). Chemicals identified from volatiles are dimethyl disulfide, dimethyl trisulfide, phenol, p-cresol, and indole; a blend of these attracted females to oviposit. Present studies investigated the effectiveness of these compounds, either in a blend or individually as potential oviposition attractants. Tests were conducted to determine the effects of gender, ovarian age, and the color and type of substrates on attraction response and oviposition of C. macellaria adults. Results showed that substrates treated with dimethyl trisulfide (DMTS) alone or the five-compound blend alone attracted significantly more gravid females than other chemicals. Black substrates treated with DMTS attracted more gravid flies than did the yellow substrates. Yellow substrates treated with indole attracted more males and nongravid females. In oviposition tests, females deposited significantly more eggs on meat-based substrates than those without meat. These findings suggest that several factors have to be considered for developing an effective oviposition attractant that should include effectiveness of individual chemicals used, the ratio of the chemicals in a blend, and their concentrations. Also, an effective trap design will need to consider using suitable color which will selectively attract gravid females.
来自废弃人工幼虫培养基以及接种了从螺旋蝇感染伤口分离出的细菌的牛血中的挥发性化学物质，会吸引嗜人瘤蝇（Cochliomyia hominivorax Coquerel）和蛆症金蝇（Cochliomyia macellaria (F.)）的孕蝇。从挥发物中鉴定出的化学物质有二甲基二硫醚、二甲基三硫醚、苯酚、对甲酚和吲哚；这些物质的混合物会吸引雌蝇产卵。目前的研究调查了这些化合物作为潜在产卵引诱剂单独或混合使用时的有效性。进行了测试以确定性别、卵巢年龄以及底物的颜色和类型对蛆症金蝇成虫的吸引反应和产卵的影响。结果表明，单独用二甲基三硫醚（DMTS）处理的底物或单独的五种化合物混合物吸引的孕蝇明显多于其他化学物质。用DMTS处理的黑色底物比黄色底物吸引更多的孕蝇。用吲哚处理的黄色底物吸引更多的雄蝇和未孕雌蝇。在产卵测试中，雌蝇在含肉底物上产的卵明显多于不含肉的底物。这些发现表明，开发一种有效的产卵引诱剂必须考虑几个因素，包括所用单一化学物质的有效性、混合物中化学物质的比例及其浓度。此外，有效的诱捕器设计需要考虑使用合适的颜色，以选择性地吸引孕蝇。
