USDA-ARS, SRU, 137 Keim Hall, UNL-East Campus, Lincoln, NE 68583-0937, USA.
J Med Entomol. 2012 Mar;49(2):293-8. doi: 10.1603/me11194.
The waste artificial larval rearing media of New World screwworms, Cochliomyia hominivorax (Coquerel) were evaluated to determine their effectiveness as oviposition attractants. Various concentrations of waste larval media resulting from rearing screwworm larvae in gel and cellulose fiber-based artificial diets tested over a 4-wk period attracted varying number of gravid screwworm flies to oviposit. Three-day-old waste medium with concentrations of 10 and 25% were most attractive to gravid female flies for oviposition and resulted in the most oviposition. One and 7-d-old wastes at all concentrations were less attractive for oviposition than the 3d-old media. The fresh (0-d-old), 14-d- and 28-d-old waste media were the least attractive substrates for oviposition. The waste from the cellulose fiber-based diet resulted in significantly more oviposition compared with waste from the gel-based diet. Microorganisms growing in the waste media probably produce metabolites that attract gravid screwworm flies to oviposit. Use of the waste products of appropriate age and dilution as oviposition substrates would enhance oviposition in mass production colony cages.
新世界螺旋蝇（Cochliomyia hominivorax（Coquerel））的废弃幼虫人工饲养基质被评估为产卵引诱物的有效性。在 4 周的时间内，测试了各种浓度的凝胶和纤维素纤维基人工饲料中饲养螺旋蝇幼虫产生的废弃幼虫培养基，吸引了不同数量的怀孕螺旋蝇产卵。3 天龄的废弃培养基浓度为 10%和 25%对怀孕雌性苍蝇产卵最具吸引力，产卵量最多。所有浓度的 1 天龄和 7 天龄废物的产卵吸引力均低于 3 天龄的废物。新鲜（0 天龄）、14 天龄和 28 天龄的废弃培养基是最不具吸引力的产卵基质。纤维素纤维基饮食产生的废物与凝胶基饮食产生的废物相比，产卵量明显更多。在废弃培养基中生长的微生物可能会产生代谢物，吸引怀孕的螺旋蝇产卵。使用适当年龄和稀释度的废物产物作为产卵基质将增强大规模生产笼中的产卵。
