CXCR4、CCR7、VEGF-C和VEGF-D表达与宫颈癌患者淋巴结转移的相关性

Association of CXCR4, CCR7, VEGF-C and VEGF-D expression with lymph node metastasis in patients with cervical cancer.

作者信息

Dai Yifei, Tong Rui, Guo Hui, Yu Tingting, Wang Chunyan

机构信息

Department of Gynaecologic Oncology, Cancer Hospital of China Medical University & Liaoning Cancer Hospital and Institute, China.

出版信息

Eur J Obstet Gynecol Reprod Biol. 2017 Jul;214:178-183. doi: 10.1016/j.ejogrb.2017.04.043. Epub 2017 Apr 27.

DOI:10.1016/j.ejogrb.2017.04.043

PMID:28535405

Abstract

OBJECTIVE

We attempted to investigate the expression of CXCR4, CCR7, VEGF-C and VEGF-D in cervical cancer specimens, and the association between CXCR4, CCR7, VEGF-C and VEGF-D expression with the clinicopathological parameters of patients with cervical cancer.

STUDY DESIGN

57 tissue microarrays including 9 normal cervical tissues and 48 cervical cancer tissues were purchased from Biomax. The association between CXCR4, CCR7, VEGF-C and VEGF-D expression with the clinicopathological parameters were evaluated. Then immunohistochemistry was used to assess the expression of CXCR4, CCR7, VEGF-C and VEGF-D in cervical cancer specimens. Finally, Spearman correlations were used for the correlation analyses between CXCR4, CCR7, VEGF-C and VEGF-D.

RESULTS

We revealed that CXCR4 expression was significantly higher in patients with squamous cell carcinomas (P=0.002) and lymph node metastasis (P=0.038), while CCR7 expression was significantly elevated in patients with lymph node metastasis (P=0.037). VEGF-C expression was markedly up-regulated in patients exhibiting FIGO stage II-III tumors (P=0.015) and lymph node metastasis (P=0.038), while VEGF-D expression was obviously increased in patients displaying FIGO stage II-III tumors (P=0.025), squamous carcinomas (P=0.017) and lymph node metastasis (P=0.037). The correlation analysis indicated that CXCR4, CCR7, VEGF-C and VEGF-D expression have a significant correlation to each other.

CONCLUSION

These results suggested that CXCR4, CCR7, VEGF-C and VEGF-D expression might have synergistic effects on the lymph node metastasis in patients with cervical cancer.

摘要

目的

我们试图研究CXCR4、CCR7、VEGF-C和VEGF-D在宫颈癌标本中的表达，以及CXCR4、CCR7、VEGF-C和VEGF-D表达与宫颈癌患者临床病理参数之间的关联。

研究设计

从Biomax购买了57个组织芯片，包括9个正常宫颈组织和48个宫颈癌组织。评估CXCR4、CCR7、VEGF-C和VEGF-D表达与临床病理参数之间的关联。然后采用免疫组织化学法评估CXCR4、CCR7、VEGF-C和VEGF-D在宫颈癌标本中的表达。最后，使用Spearman相关性分析CXCR4、CCR7、VEGF-C和VEGF-D之间的相关性。

结果

我们发现，CXCR4表达在鳞状细胞癌患者中显著更高（P=0.002），在有淋巴结转移的患者中也显著更高（P=0.038），而CCR7表达在有淋巴结转移的患者中显著升高（P=0.037）。VEGF-C表达在国际妇产科联盟（FIGO）II-III期肿瘤患者中明显上调（P=0.015），在有淋巴结转移的患者中也明显上调（P=0.038），而VEGF-D表达在FIGO II-III期肿瘤患者（P=0.025）、鳞状细胞癌患者（P=0.017）和有淋巴结转移的患者（P=0.037）中明显增加。相关性分析表明，CXCR4、CCR7、VEGF-C和VEGF-D表达之间存在显著相关性。