Dai Yifei, Tong Rui, Guo Hui, Yu Tingting, Wang Chunyan
Department of Gynaecologic Oncology, Cancer Hospital of China Medical University & Liaoning Cancer Hospital and Institute, China.
Department of Gynaecologic Oncology, Cancer Hospital of China Medical University & Liaoning Cancer Hospital and Institute, China.
Eur J Obstet Gynecol Reprod Biol. 2017 Jul;214:178-183. doi: 10.1016/j.ejogrb.2017.04.043. Epub 2017 Apr 27.
We attempted to investigate the expression of CXCR4, CCR7, VEGF-C and VEGF-D in cervical cancer specimens, and the association between CXCR4, CCR7, VEGF-C and VEGF-D expression with the clinicopathological parameters of patients with cervical cancer.
57 tissue microarrays including 9 normal cervical tissues and 48 cervical cancer tissues were purchased from Biomax. The association between CXCR4, CCR7, VEGF-C and VEGF-D expression with the clinicopathological parameters were evaluated. Then immunohistochemistry was used to assess the expression of CXCR4, CCR7, VEGF-C and VEGF-D in cervical cancer specimens. Finally, Spearman correlations were used for the correlation analyses between CXCR4, CCR7, VEGF-C and VEGF-D.
We revealed that CXCR4 expression was significantly higher in patients with squamous cell carcinomas (P=0.002) and lymph node metastasis (P=0.038), while CCR7 expression was significantly elevated in patients with lymph node metastasis (P=0.037). VEGF-C expression was markedly up-regulated in patients exhibiting FIGO stage II-III tumors (P=0.015) and lymph node metastasis (P=0.038), while VEGF-D expression was obviously increased in patients displaying FIGO stage II-III tumors (P=0.025), squamous carcinomas (P=0.017) and lymph node metastasis (P=0.037). The correlation analysis indicated that CXCR4, CCR7, VEGF-C and VEGF-D expression have a significant correlation to each other.
These results suggested that CXCR4, CCR7, VEGF-C and VEGF-D expression might have synergistic effects on the lymph node metastasis in patients with cervical cancer.
我们试图研究CXCR4、CCR7、VEGF-C和VEGF-D在宫颈癌标本中的表达，以及CXCR4、CCR7、VEGF-C和VEGF-D表达与宫颈癌患者临床病理参数之间的关联。
从Biomax购买了57个组织芯片，包括9个正常宫颈组织和48个宫颈癌组织。评估CXCR4、CCR7、VEGF-C和VEGF-D表达与临床病理参数之间的关联。然后采用免疫组织化学法评估CXCR4、CCR7、VEGF-C和VEGF-D在宫颈癌标本中的表达。最后，使用Spearman相关性分析CXCR4、CCR7、VEGF-C和VEGF-D之间的相关性。
我们发现，CXCR4表达在鳞状细胞癌患者中显著更高（P=0.002），在有淋巴结转移的患者中也显著更高（P=0.038），而CCR7表达在有淋巴结转移的患者中显著升高（P=0.037）。VEGF-C表达在国际妇产科联盟（FIGO）II-III期肿瘤患者中明显上调（P=0.015），在有淋巴结转移的患者中也明显上调（P=0.038），而VEGF-D表达在FIGO II-III期肿瘤患者（P=0.025）、鳞状细胞癌患者（P=0.017）和有淋巴结转移的患者（P=0.037）中明显增加。相关性分析表明，CXCR4、CCR7、VEGF-C和VEGF-D表达之间存在显著相关性。
这些结果表明，CXCR4、CCR7、VEGF-C和VEGF-D表达可能对宫颈癌患者的淋巴结转移具有协同作用。
