Cai X Y, Lu Y, Tang C, Lin X J, Ye J H, Li W N, He Z X, Li F F
Department of Rheumatology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China.
Zhonghua Yi Xue Za Zhi. 2017 May 23;97(19):1491-1495. doi: 10.3760/cma.j.issn.0376-2491.2017.19.013.
To analyze peripheral blood interleukin-6 (IL-6) promoter DNA methylation status and its clinical significance in patients with systemic lupus erythematosus (SLE). Blood samples of 41 adult patients with SLE and 20 healthy controls were collected.The methylation status of IL-6 promoter was determined by methylation specific polymerase chain reaction (MSP). The IL-6 expression was detected by real-time PCR.Correlations between IL-6 promoter methylation status and clinical features or laboratory findings in patients with SLE were analyzed. The levels of IL-6 mRNA were significantly higher in peripheral blood of SLE.DNA methylation levels of IL-6 promoter were reduced in SLE patients as compared with healthy controls.The methylation status and expression of IL-6 in peripheral blood reflected the levels in peripheral blood mononuclear cells (PBMCs). Significantly positive correlation was found between IL-6 hypomethylation and renal disorder, as well as hypocomplementemia in patients with SLE. Hypomethylation of interleukin-6 promoter in peripheral blood might be involved in the etiology of SLE.
分析系统性红斑狼疮（SLE）患者外周血白细胞介素-6（IL-6）启动子DNA甲基化状态及其临床意义。收集41例成年SLE患者和20例健康对照者的血样。采用甲基化特异性聚合酶链反应（MSP）检测IL-6启动子的甲基化状态。通过实时PCR检测IL-6表达。分析SLE患者IL-6启动子甲基化状态与临床特征或实验室检查结果之间的相关性。SLE患者外周血中IL-6 mRNA水平显著升高。与健康对照相比，SLE患者IL-6启动子的DNA甲基化水平降低。外周血中IL-6的甲基化状态和表达反映了外周血单个核细胞（PBMCs）中的水平。SLE患者中IL-6低甲基化与肾脏疾病以及低补体血症之间存在显著正相关。外周血白细胞介素-6启动子的低甲基化可能参与了SLE的发病机制。
