硬骨鱼（条石鲷，斑石鲷）中CD2相关蛋白基因的首次报道：对几种病原体感染后免疫反应的研究
The first report of CD2 associated protein gene, in a teleost (Rock bream, Oplegnathus fasciatus): An investigation of the immune response upon infection with several pathogens.
作者信息
Cho Dong-Hee, Bae Jin-Sol, Jeong Ji-Min, Han Hyun-Ja, Lee Deok Chan, Cho Mi Young, Jung Sung Hee, Kim Do-Hyung, Park Chan-Il
机构信息
Department of Marine Biology & Aquaculture, College of Marine Science, Gyeongsang National University, 455, Tongyeong, 650-160, Republic of Korea.
Pathology Research Division, National Institute of Fisheries Science, 408-1 Sirang-ri, Gijang-up, Gijang-gun, Busan 46083, Republic of Korea.
出版信息
Fish Shellfish Immunol. 2017 Aug;67:1-6. doi: 10.1016/j.fsi.2017.05.045. Epub 2017 May 20.
CD2 is expressed on the surfaces of virtually all T cells and natural killer (NK) cells. In mammals, the CD2 molecule is 50 kDa. The cytoplasmic tail of CD2 interacts with CD2-associated protein (CD2AP), which plays an important role in mediating the trigger signal in outer magnetic pole cells. In this study, we identified CD2AP from rock bream and investigated its gene expression. The ORF of CD2AP (1950 bp) encodes 650 amino acids (aa). CD2AP has a Src homology 3 (SH3) domain. Quantitative real-time PCR analysis revealed that CD2AP shows higher expression in the gills and skin. Under experimental challenge, CD2AP gene expression was increased as relative to the control after 7 days. This result will improve our understanding of blood vessels in teleost fish, and will provide a basis for the study of CD2-related genes.
CD2几乎在所有T细胞和自然杀伤（NK）细胞表面表达。在哺乳动物中，CD2分子为50千道尔顿。CD2的细胞质尾巴与CD2相关蛋白（CD2AP）相互作用，CD2AP在介导外磁极细胞中的触发信号方面发挥重要作用。在本研究中，我们从条石鲷中鉴定出CD2AP并研究了其基因表达。CD2AP的开放阅读框（1950 bp）编码650个氨基酸（aa）。CD2AP具有一个Src同源3（SH3）结构域。实时定量PCR分析显示，CD2AP在鳃和皮肤中表达较高。在实验性刺激下，7天后CD2AP基因表达相对于对照组增加。这一结果将增进我们对硬骨鱼类血管的理解，并为CD2相关基因的研究提供基础。
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