酚妥拉明对血小板聚集、血栓素B2生成及释放反应的影响。

Influence of phentolamine on platelet aggregation, thromboxane B2 production and release reaction.

作者信息

Anfossi G, Trovati M, Mularoni E, Lanzio M, Massucco P, Emanuelli G

机构信息

Institute of Internal Medicine, University of Turin, Ospedale S. Luigi Gonzaga-Orbassano, Torino, Italy.

出版信息

Arch Int Pharmacodyn Ther. 1988 Nov-Dec;296:144-54.

PMID:2853612

Abstract

In this study the in vitro influence of phentolamine on platelet aggregating responses, thromboxane B2 (TxB2) production and intraplatelet cyclic AMP (cAMP) content has been investigated. The drug exerts a dose-dependent inhibitory effect on aggregating response to ADP, PAF, collagen, thrombin, sodium arachidonate and ionophore A 23187. Inhibiting phentolamine concentrations prevent also platelet release reaction and TxB2 synthesis. No significant influence on intraplatelet cAMP levels has been observed. The pre-incubation with low concentrations of ionophore A 23187 overcomes phentolamine inhibition of collagen-induced platelet aggregation. Our results provide evidence that phentolamine modulates the human platelet function and that its effects could also be related to a decrease of Ca++ availability.

摘要

在本研究中，已对酚妥拉明对血小板聚集反应、血栓素B2（TxB2）生成及血小板内环磷酸腺苷（cAMP）含量的体外影响进行了研究。该药物对ADP、PAF、胶原、凝血酶、花生四烯酸钠及离子载体A 23187诱导的聚集反应具有剂量依赖性抑制作用。抑制性酚妥拉明浓度也可阻止血小板释放反应及TxB2合成。未观察到对血小板内cAMP水平有显著影响。低浓度离子载体A 23187预孵育可克服酚妥拉明对胶原诱导的血小板聚集的抑制作用。我们的结果证明，酚妥拉明可调节人类血小板功能，其作用也可能与Ca++可用性降低有关。