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Catalytic Intermediate Crystal Structures of Cysteine Desulfurase from the Archaeon NA1.

作者信息

Ho Thien-Hoang, Huynh Kim-Hung, Nguyen Diem Quynh, Park Hyunjae, Jung Kyoungho, Sur Bookyo, Ahn Yeh-Jin, Cha Sun-Shin, Kang Lin-Woo

机构信息

Department of Biological Sciences, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea.

Department of Life Science, Sangmyung University, 7 Hongji-dong, Jongno-gu, Seoul 03016, Republic of Korea.

出版信息

Archaea. 2017 Apr 24;2017:5395293. doi: 10.1155/2017/5395293. eCollection 2017.

DOI:10.1155/2017/5395293
PMID:28536498
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5426080/
Abstract

NA1 is an anaerobic archaeon usually found in a deep-sea hydrothermal vent area, which can use elemental sulfur (S) as a terminal electron acceptor for energy. Sulfur, essential to many biomolecules such as sulfur-containing amino acids and cofactors including iron-sulfur cluster, is usually mobilized from cysteine by the pyridoxal 5'-phosphate- (PLP-) dependent enzyme of cysteine desulfurase (CDS). We determined the crystal structures of CDS from NA1 (ToCDS), which include native internal aldimine (NAT), gem-diamine (GD) with alanine, internal aldimine structure with existing alanine (IAA), and internal aldimine with persulfide-bound Cys356 (PSF) structures. The catalytic intermediate structures showed the dihedral angle rotation of Schiff-base linkage relative to the PLP pyridine ring. The ToCDS structures were compared with bacterial CDS structures, which will help us to understand the role and catalytic mechanism of ToCDS in the archaeon NA1.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d68/5426080/f8799b168159/ARCHAEA2017-5395293.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d68/5426080/76c3d743d4b3/ARCHAEA2017-5395293.sch.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d68/5426080/66a209902cc0/ARCHAEA2017-5395293.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d68/5426080/b4dbd1edc418/ARCHAEA2017-5395293.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d68/5426080/232966249bf3/ARCHAEA2017-5395293.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d68/5426080/1f9e202dc58c/ARCHAEA2017-5395293.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d68/5426080/c0f70b808c88/ARCHAEA2017-5395293.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d68/5426080/f8799b168159/ARCHAEA2017-5395293.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d68/5426080/76c3d743d4b3/ARCHAEA2017-5395293.sch.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d68/5426080/66a209902cc0/ARCHAEA2017-5395293.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d68/5426080/b4dbd1edc418/ARCHAEA2017-5395293.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d68/5426080/232966249bf3/ARCHAEA2017-5395293.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d68/5426080/1f9e202dc58c/ARCHAEA2017-5395293.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d68/5426080/c0f70b808c88/ARCHAEA2017-5395293.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d68/5426080/f8799b168159/ARCHAEA2017-5395293.006.jpg

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