生物素标记的糖基磷脂酰肌醇（GPI）锚定核心聚糖的合成及其在研究GPI与成孔细菌毒素相互作用中的应用。
Synthesis of biotin-labelled core glycans of GPI anchors and their application in the study of GPI interaction with pore-forming bacterial toxins.
作者信息
Gao Jian, Zhou Zhifang, Guo Jiatong, Guo Zhongwu
机构信息
Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, Michigan 48202, USA.
出版信息
Chem Commun (Camb). 2017 Jun 6;53(46):6227-6230. doi: 10.1039/c7cc03056h.
Abstract
A convergent strategy was developed for the first-time synthesis of biotin-labeled GPI core glycans. These GPI conjugates are useful for various biological studies showcased by their application in the scrutiny of pore-forming bacterial toxin-GPI interaction, revealing that the phosphate group at the GPI inositol 1-O-position had a significant impact on GPI-toxin binding.
摘要
开发了一种收敛策略，用于首次合成生物素标记的糖基磷脂酰肌醇（GPI）核心聚糖。这些GPI缀合物可用于各种生物学研究，通过其在检查成孔细菌毒素与GPI相互作用中的应用得到了证明，这表明GPI肌醇1-O位的磷酸基团对GPI-毒素结合有显著影响。
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