自发性高血压大鼠中乳头集合管对血管活性激素的反应性

Papillary collecting tubule responsiveness to vasoactive hormones in spontaneously hypertensive rats.

作者信息

Sato M, Abe K, Takeuchi K, Yasujima M, Fang S, Kanazawa M, Yoshida K, Yoshinaga K

机构信息

Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.

出版信息

J Hypertens Suppl. 1988 Dec;6(4):S476-8. doi: 10.1097/00004872-198812040-00150.

DOI:10.1097/00004872-198812040-00150

PMID:2853755

Abstract

Renal papillary collecting tubule (RPCT) hormone responsiveness was compared between cultured RPCT cells from spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY). Unstimulated cells from 4-week-old SHR produced less prostaglandin E2 (PGE2) and cyclic (c)AMP than comparable cells from WKY, while cells from both stains synthesized similar amounts of PGE2 after stimulation with arachidonate, A23187 or bradykinin and similar amounts of cAMP after stimulation with vasopressin or PGE2. There was no difference in basal or stimulated levels of cyclic (c)GMP between the strains. In RPCT cells from 16-week-old rats, basal levels of cAMP, cGMP and PGE2 were significantly lower than those from 4-week-old rats, but they did not differ between the strains. These results suggest that RPCT cells of SHR and WKY at the post-weaning period may differ in the metabolism of PGE2 and cAMP. This difference may be attributed to the possible defect in arachidonate availability in SHR.

摘要

比较了自发性高血压大鼠（SHR）和Wistar-Kyoto大鼠（WKY）培养的肾乳头集合管（RPCT）细胞对激素的反应性。4周龄SHR的未刺激细胞产生的前列腺素E2（PGE2）和环磷酸腺苷（cAMP）比WKY的同类细胞少，而两种品系的细胞在用花生四烯酸、A23187或缓激肽刺激后合成的PGE2量相似，在用血管加压素或PGE2刺激后合成的cAMP量相似。品系间环磷酸鸟苷（cGMP）的基础水平或刺激水平没有差异。在16周龄大鼠的RPCT细胞中，cAMP、cGMP和PGE2的基础水平显著低于4周龄大鼠，但品系间没有差异。这些结果表明，断奶后SHR和WKY的RPCT细胞在PGE2和cAMP代谢方面可能存在差异。这种差异可能归因于SHR中花生四烯酸可用性可能存在的缺陷。