干扰素-γ 通过调节 Fas/FasL 信号通路影响白血病细胞凋亡。

Interferon-γ affects leukemia cell apoptosis through regulating Fas/FasL signaling pathway.

机构信息

Department of Hematology, The Affiliated Chaohu Hospital of Anhui Medical University, Chaohu, Anhui, China.

出版信息

Eur Rev Med Pharmacol Sci. 2017 May;21(9):2244-2248.

Abstract

OBJECTIVE

Imbalance of hematopoietic cell proliferation and apoptosis is one of the major causes of leukemia. Enhanced cell proliferation and reduced apoptosis lead to hemocytes accumulation. Fas/FasL signaling pathway promotes cell apoptosis. This study investigated the impact of interferon γ (IFN-γ) on chronic myelogenous leukemia cell proliferation and apoptosis to elucidate its interaction with Fas/FasL signaling pathway.

PATIENTS AND METHODS

Leukemia K562 cells were routinely cultivated and treated with 10 U/ml, 100 U/ml, and 1000 U/ml interferon for 12 h, 24 h, and 48 h, respectively. MTT assay was applied to test cell proliferation. TUNEL assay was adopted to determine cell apoptosis. Western blot was selected to detect Fas/FasL expression.

RESULTS

Different concentrations of IFN-γ inhibited cell proliferation at various time points. IFN-γ at 1000 U/ml treatment for 48 h exhibited the strongest suppressive effect on cell proliferation (p < 0.05). IFN-γ intervention enhanced K562 cell apoptosis with concentration and time dependence (p < 0.05). Fas and FasL proteins expressions upregulated after treated by IFN-γ following dose elevation and time extension (p < 0.05).

CONCLUSIONS

IFN-γ inhibits leukemia K562 cell proliferation and promotes cell apoptosis via facilitating Fas and FasL proteins expressions.

摘要

目的

造血细胞增殖和凋亡失衡是白血病的主要原因之一。增强的细胞增殖和减少的细胞凋亡导致血细胞积聚。Fas/FasL 信号通路促进细胞凋亡。本研究探讨了干扰素 γ（IFN-γ）对慢性髓系白血病细胞增殖和凋亡的影响，以阐明其与 Fas/FasL 信号通路的相互作用。

患者和方法

常规培养白血病 K562 细胞，并分别用 10 U/ml、100 U/ml 和 1000 U/ml 的干扰素处理 12 h、24 h 和 48 h。MTT 法检测细胞增殖，TUNEL 法检测细胞凋亡，Western blot 法检测 Fas/FasL 表达。

结果

不同浓度的 IFN-γ 在不同时间点抑制细胞增殖。IFN-γ 在 1000 U/ml 处理 48 h 时对细胞增殖的抑制作用最强（p<0.05）。IFN-γ 干预增强了 K562 细胞凋亡，具有浓度和时间依赖性（p<0.05）。随着剂量的增加和时间的延长，IFN-γ 处理后 Fas 和 FasL 蛋白表达上调（p<0.05）。