Goverde H J, Pesman G J, Smals A G
Department of Medical Biology, Catholic University, Nijmegen, The Netherlands.
Neuropeptides. 1988 Oct;12(3):125-30. doi: 10.1016/0143-4179(88)90042-x.
The ability of two pro-opiomelanocortin-derived peptides, the melanotropin potentiating factor (MPF) and beta-endorphin (beta EP), to affect corticosteroid production was studied in purified isolated rat adrenal cells. Addition of MPF or beta EP, in doses from 5 pg to 5 micrograms, alone did not result in a corticosterone production. Furthermore, no effect of MPF or beta EP in doses from 5 pg to as high as 5 micrograms for both peptides upon the ACTH or alpha-MSH-induced corticosteroidogenesis was observed (p greater than 0.1). It is concluded that both MPF and beta EP do not influence the steroidogenic activity in the adrenal gland. Use of these peptides for discrimination of the ACTH/alpha-MSH receptor interactions is suggested.
在纯化的离体大鼠肾上腺细胞中，研究了两种源自阿片促黑皮质素原的肽——促黑素增强因子（MPF）和β-内啡肽（βEP）影响皮质类固醇生成的能力。单独添加剂量为5皮克至5微克的MPF或βEP，并不会导致皮质酮的生成。此外，对于这两种肽，在剂量从5皮克到高达5微克的情况下，未观察到MPF或βEP对促肾上腺皮质激素（ACTH）或α-促黑素（α-MSH）诱导的皮质类固醇生成有影响（p>0.1）。得出的结论是，MPF和βEP均不影响肾上腺中的类固醇生成活性。建议使用这些肽来区分ACTH/α-MSH受体相互作用。
