血管活性肠肽（VIP）和生长激素释放因子（GRF）与促肾上腺皮质激素释放因子（CRF）在体外对促肾上腺皮质激素分泌的相互作用。

Interaction of vasoactive intestinal peptide (VIP) and growth hormone releasing factor (GRF) with corticotropin releasing factor (CRF) on corticotropin secretion in vitro.

作者信息

Léonard J F, Bluet-Pajot M T, Oliver C, Kordon C

机构信息

Unité 159 INSERM, Paris, France.

出版信息

Neuropeptides. 1988 Oct;12(3):131-3. doi: 10.1016/0143-4179(88)90043-1.

DOI:10.1016/0143-4179(88)90043-1

PMID:2853835

Abstract

The possible interaction of VIP and GRF with CRF on ACTH release was examined by using superfused rat anterior pituitary fragments. ACTH was measured in effluent medium samples by RIA. Increasing concentrations of VIP and GRF (10(-8) to 10(-6)M) had no effect on spontaneous ACTH secretion but potentiated CRF (10(-10)M) induced ACTH release in a dose dependent manner. The high concentrations of VIP or GRF required to produce the effect suggest that both the peptides could interfere with a common unidentified binding site on corticotrophs or stimulate a second messenger by a paracrine action.

摘要

通过使用超灌流大鼠垂体前叶碎片，研究了血管活性肠肽（VIP）和生长激素释放因子（GRF）与促肾上腺皮质激素释放因子（CRF）在促肾上腺皮质激素（ACTH）释放方面可能存在的相互作用。采用放射免疫分析法（RIA）测定流出培养基样本中的促肾上腺皮质激素。血管活性肠肽和生长激素释放因子浓度增加（10⁻⁸至10⁻⁶M）对促肾上腺皮质激素的自发分泌无影响，但能以剂量依赖方式增强促肾上腺皮质激素释放因子（10⁻¹⁰M）诱导的促肾上腺皮质激素释放。产生该效应所需的血管活性肠肽或生长激素释放因子的高浓度表明，这两种肽可能会干扰促肾上腺皮质细胞上一个尚未确定的共同结合位点，或通过旁分泌作用刺激第二信使。