Dooley D J, Lickert M, Lupp A, Osswald H
Department of Pharmacology, Gödecke Research Institute, Freiburg, F.R.G.
Neurosci Lett. 1988 Nov 11;93(2-3):318-23. doi: 10.1016/0304-3940(88)90102-4.
Potential age-related changes in L- and N-type voltage-sensitive calcium channels (L- and N-VSCCs) were assessed by the in vitro binding of [3H]isradipine ([3H]ISR, 150 pM) and [125]omega-conotoxin GVIA ([125I]omega-CT, 4 pM) to membranes prepared from discrete central nervous system regions of 0.5-, 2-, and 18-month-old rats. The rank orders of [3H]ISR and [125I]omega-CT binding, although differing, indicated that the highest binding was in neocortex, corpus striatum, and hippocampus; radioligand binding was generally not affected by the variable of age. These results suggest that the nonidentical [3H]ISR and [125I]omega-CT binding sites are concentrated in those regions characterized by high densities of synaptic connections, and that these sites, as presumed components of L- and N-VSCCs, are relatively stable during the aging process.
通过[3H]异搏定（[3H]ISR，150 pM）和[125I]ω-芋螺毒素GVIA（[125I]ω-CT，4 pM）与从0.5个月、2个月和18个月大的大鼠离散中枢神经系统区域制备的膜进行体外结合，评估了L型和N型电压敏感性钙通道（L-VSCCs和N-VSCCs）潜在的年龄相关变化。[3H]ISR和[125I]ω-CT结合的排序虽然不同，但表明最高结合发生在新皮质、纹状体和海马体；放射性配体结合一般不受年龄变量的影响。这些结果表明，不同的[3H]ISR和[125I]ω-CT结合位点集中在以高密度突触连接为特征的区域，并且这些位点作为L-VSCCs和N-VSCCs的假定组成部分，在衰老过程中相对稳定。
