Yang Yafei, Yang Jin, Chen Lin, Hu Jianyun, Xing Shasha, Amend Bastian, Stenzl Arnulf, Wei Xin, Hu Haifeng
Department of Urology, Affiliated Hospital of Chengdu University, Chengdu, Sichuan, China (mainland).
Central Laboratory, Affiliated Hospital of Chengdu University, Chengdu, Sichuan, China (mainland).
Med Sci Monit. 2017 May 24;23:2500-2507. doi: 10.12659/msm.904760.
BACKGROUND The abdominal straining pattern can act as a novel parameter for improving the prediction of bladder outlet obstruction (BOO). To preserve detrusor function in the early stage of urinary system impairment, such as BOO, we establish a novel method for cystometry and Intra-abdominal pressure (IAP) assessments in rodents without cystostomy. MATERIAL AND METHODS Twenty mice and rats were divided into three groups (control, sham-operated and BOO group) respectively. The cystometry and IAP assessments were measured by the pediatric venous indwelling sheath and coronary dilatation catheter connected to Laborie urodynamic system on postoperative day 7. Data was collected simultaneously through urethra and rectum in each group. In addition, bladder histology was assessed to confirm BOO. RESULTS The novel method can collect the urodynamic parameters successfully, including the BLPP, IAP, MBC, etc. IAP was elevated in BOO rats, but no significantly difference was found between the sham-operated rats and the control rats. The hypertrophy of detrusor muscle in bladder section was observed by Masson trichrome staining in BOO group compared with other groups. CONCLUSIONS Our novel method based on innovative research implement for cystometry and IAP assessments in rodents is a reliable and replicable approach for evaluating the lower urinary tract function. Especially it provides detailed information to evaluate lower urinary tract structures and function in the early stage of BOO.
背景 腹部用力模式可作为改善膀胱出口梗阻（BOO）预测的新参数。为了在泌尿系统损伤的早期阶段（如BOO）保留逼尿肌功能，我们建立了一种在啮齿动物中无需膀胱造口术进行膀胱测压和腹内压（IAP）评估的新方法。
材料与方法 将20只小鼠和大鼠分别分为三组（对照组、假手术组和BOO组）。术后第7天，通过连接到Laborie尿动力学系统的小儿静脉留置鞘管和冠状动脉扩张导管测量膀胱测压和IAP评估。每组通过尿道和直肠同时收集数据。此外，评估膀胱组织学以确认BOO。
结果 该新方法能够成功收集尿动力学参数，包括膀胱漏尿点压（BLPP）、IAP、膀胱最大容量（MBC）等。BOO大鼠的IAP升高，但假手术大鼠和对照大鼠之间未发现显著差异。与其他组相比，BOO组通过Masson三色染色观察到膀胱切片中逼尿肌肥大。
结论 我们基于创新研究工具在啮齿动物中进行膀胱测压和IAP评估的新方法是一种可靠且可重复的评估下尿路功能的方法。特别是它为评估BOO早期的下尿路结构和功能提供了详细信息。
