Dybvig K, Alderete J
Department of Comparative Medicine, University of Alabama, Birmingham 35294.
Plasmid. 1988 Jul;20(1):33-41. doi: 10.1016/0147-619x(88)90005-4.
A procedure for transformation of the murine pathogen Mycoplasma pulmonis with plasmid pAM120 was developed. This plasmid replicates in Escherichia coli and contains the gram-positive transposon Tn916. The transformation protocol also proved effective for the swine pathogen Mycoplasma hyorhinis. The tetracycline resistance determinant of Tn916 was expressed in transformed myocoplasma cells, and Tn916 was found inserted into numerous sites in the recipient chromosomes of M. pulmonis and M. hyorhinis, indicating that transposition had occurred. Interestingly, some transformants of M. pulmonis and M. hyorhinis contained cointegrate structures which apparently had a complete copy of the entire donor plasmid (pAM120) inserted into the recipient chromosome. Subsequent transposition of inserted Tn916 was observed in passaged clones of transformed M. pulmonis.
已开发出一种用质粒pAM120转化鼠病原体肺支原体的方法。该质粒在大肠杆菌中复制，并含有革兰氏阳性转座子Tn916。该转化方案对猪病原体猪鼻支原体也证明有效。Tn916的四环素抗性决定簇在转化的支原体细胞中表达，并且发现Tn916插入到肺支原体和猪鼻支原体受体染色体的多个位点，表明发生了转座。有趣的是，肺支原体和猪鼻支原体的一些转化体含有共整合结构，其中显然有整个供体质粒（pAM120）的完整拷贝插入到受体染色体中。在转化的肺支原体传代克隆中观察到插入的Tn916的后续转座。
